α-Adrenergic regulation of the activity of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy
α-Adrenergic regulation of the activity of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy
复制标题
部分肝切除术后肝再生过程中胸苷酸合成酶和胸苷激酶活性的α-肾上腺素调节
DOI：
10.1016/0014-2999(85)90380-2
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发表时间：
1985
影响因子：
5
通讯作者：
S. Kojo
中科院分区：
文献类型：
--
作者：
R. Nakata;I. Tsukamoto;M. Nanme;S. Makino;M. Miyoshi;S. Kojo
在给予α-肾上腺素能受体拮抗剂（phenoxybenzamine和酚妥拉明）或肾上腺素能神经元阻滞剂（guanethidine和利血平）的大鼠肝脏再生过程中，肝胸腺苷酸合成酶和胸腺苷激酶活性的增加（分别通过新生途径和挽救途径催化胸腺苷酸的形成）被显著抑制。β-肾上腺素受体拮抗剂（心得安）或抗胆碱能剂（硝酸甲酯阿托品）没有观察到这些抑制作用。胸腺苷合成酶活性的增加与肝脏DNA含量的增加密切相关。由此可见，儿茶酚胺调节了肝再生DNA合成的关键酶胸腺苷酸合成酶和胸腺苷激酶活性的升高。同时也提示交感神经在肝脏再生中起重要作用。
The increases in activity of hepatic thymidylate synthetase and of thymidine kinase, which catalyze the formation of thymidylate via the de novo and salvage pathways, respectively, were significantly suppressed during liver regeneration in rats which had been givenα-adrenoceptor antagonists (phenoxybenzamine and phentolamine) or adrenergic neuron blockers (guanethidine and reserpine). These suppressions were not observed with aβ-adrencoceptor antagonist (propranolol), or an anticholinergic agent (atropine methyl nitrate). The rise in the activity of the thymidylate-synthesizing enzymes was closely correlated with the increase in the DNA content of the liver. It is concluded that catecholamine regulates the increase in the activity of thymidylate synthetase and thymidine kinase, which are key enzymes in DNA synthesis in regenerating liver. It is also suggested that sympathetic nerves play an important role in liver regeneration.