Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79
Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79
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新型细菌群 SAAS79 对霉菌毒素伏马菌素 B1 的生物降解
DOI：
10.1007/s00253-019-09979-6
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发表时间：
2019
影响因子：
5
通讯作者：
Wang Jianhua
中科院分区：
文献类型：
--
作者：
Zhao Zhiyong;Zhang Yanmei;Gong Andong;Liu Na;Chen Shanshan;Zhao Xiaoyan;Li Xiaobei;Chen Lei;Zhou Changyan;Wang Jianhua
伏马菌素B1（FB 1）分布广泛，对人体健康具有潜在的危害，是粮食及其制品中的一个重要的食品安全问题。然而，据报道，只有少数微生物能有效降解FB 1。在本研究中，具有高度FB 1降解活性的细菌财团SAAS 79从废蘑菇堆肥中分离。结合16 S rDNA序列分析，确定假单胞菌属为降解FB 1的关键成员。在pH 5-7、温度28-35 °C条件下培养24 h，该菌群对10 μg/mL的FB 1的降解率可达90%以上。细胞内的酶对FB 1有降解作用，在3 h内降解率可达90%。此外，液相色谱-飞行时间质谱（LC-TOF/MS）分析鉴定了FB 1的两种降解产物，与母体FB 1相比，它们对猴肾细胞（MARC-145）的毒性显著降低（p< 0.05）。总体而言，SAAS 79及其粗酶可能是饲料和食品工业中FB 1去污的潜在选择。同时，该菌群为酶解解毒剂的开发提供了新的基因来源。
Fumonisin B1 (FB1) contamination in cereals and cereal products remains an important aspect of food safety because of its wide distribution and the potential health hazard. However, only a few microorganisms have been reported to effectively degrade FB1. In this present study, a bacterial consortium SAAS79 with highly FB1-degrading activity was isolated from the spent mushroom compost. The combination of antibiotic-driven selection and 16S rDNA sequencing identified thePseudomonasgenus as the key FB1-degrading member. The microbial consortium could degrade more than 90% of 10 μg/mL FB1 after incubation for 24 h at pH of 5–7 and temperature of 28–35 °C. The enzymes from the intracellular space were proved to be responsible for FB1 degradation, which eliminated about 90% of 10 μg/mL FB1 in 3 h. Besides, liquid chromatography time-of-flight mass spectrometry (LC-TOF/MS) analysis identified two degradation products of FB1, and their toxicity on the monkey kidney cells (MARC-145) was significantly lower (p< 0.05) compared with the parent FB1. Overall, the consortium SAAS79 and its crude enzymes may be a potential choice for the decontamination of FB1 in the feed and food industry. Also, the bacterial consortium provides a new source of genes for the development of enzymatic detoxification agent.