Amelogenin Exon 5 Peptide Promotes Cell Proliferation and Osteogenic Differentiation in Human Dental Pulp Stem Cells
Amelogenin Exon 5 Peptide Promotes Cell Proliferation and Osteogenic Differentiation in Human Dental Pulp Stem Cells
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DOI：
10.3390/app9204425
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发表时间：
2019-10-01
影响因子：
2.7
通讯作者：
Umeda, Makoto
中科院分区：
文献类型：
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作者：
Kato, Hirohito;Taguchi, Yoichiro;Umeda, Makoto
釉原蛋白是一种由多种分子大小的蛋白质和氨基酸组成的复杂的釉基质蛋白，它是一种生物活性物质，具有生物活性，是一种生物活性物质。釉原蛋白的剪接形式被鉴定为包括外显子2、3、5、6和7。然而，釉原蛋白外显子5在牙髓中的生物学功能尚不清楚。我们设计了一个人工合成的釉原蛋白外显子5编码肽（SP），这是基于一种蛋白质产生的细胞在响应釉基质衍生物（EMD）。本研究探讨了SP对牙髓干细胞成骨的促进作用及其信号通路。DPSC是维持牙髓组织稳态的重要细胞。将DPSCs与SP共同培养，观察其对细胞增殖和成骨分化的影响。我们还研究了丝裂原活化蛋白激酶（MAPK）信号通路。SP能显著促进细胞增殖和成骨分化的表达。此外，SP还促进MAPK信号通路的表达。因此，釉原蛋白外显子5可能有助于盖髓。
Featured ApplicationThe synthetic peptide of amelogenin exon 5 might be a useful application to the pulp capping treatment.Abstract Amelogenin is a complex enamel matrix protein that consists of various molecular-size proteins and amino acids. A spliced form of amelogenin was identified that included exons 2, 3, 5, 6, and 7. However, the biological function of amelogenin exon 5 on dental pulp remains unknown. We designed a synthetic amelogenin exon 5 encoded peptide (SP), which was based on a protein produced by cells in response to the enamel matrix derivative (EMD). We investigated the effect of the SP on potentiation of osteogenesis and its signal pathway in dental pulp stem cells (DPSCs). DPSCs are an important cell for pulp tissue homeostasis. DPSCs were cultured with SP to examine the effect of cell proliferation and osteogenic differentiation. We also investigated the mitogen-activated protein kinase (MAPK) signaling pathway. SP significantly enhanced cell proliferation and the expression of osteogenic differentiation. Moreover, SP promoted the expression of the MAPK signaling pathway. Therefore, amelogenin exon 5 might contribute to dental pulp capping.