Quantitative proteomics links metabolic pathways to specific developmental stages of the plant-pathogenic oomycete Phytophthora capsici.
Quantitative proteomics links metabolic pathways to specific developmental stages of the plant-pathogenic oomycete Phytophthora capsici.
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DOI：
10.1111/mpp.12406
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发表时间：
2017-04
影响因子：
4.9
通讯作者：
Zhili Pang;V. Srivastava;Xili Liu;V. Bulone
中科院分区：
文献类型：
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作者：
Zhili Pang;V. Srivastava;Xili Liu;V. Bulone
辣椒疫霉（Phytophthora capsici）是一种严重危害世界各地蔬菜生产的病原菌。它的无性繁殖在田间病害的快速繁殖和传播中起着重要作用。进行了一项全球蛋白质组学研究，以比较辣椒疫霉的两个关键无性生活阶段，即菌丝体和孢囊，以确定阶段特异性的生化过程。使用定性和定量蛋白质组学共鉴定了1200种蛋白质。还通过定量实时聚合酶链反应分析了一些富集蛋白的转录丰度。73种蛋白质在菌丝和孢囊之间表现出不同的丰度水平。菌丝体中富集的蛋白质主要与糖酵解、三羧酸（或柠檬酸）循环和戊糖磷酸途径相关，为细胞构件的生物合成和菌丝生长提供所需的能量。相反，在包囊中占主导地位的蛋白质基本上参与脂肪酸降解，这表明病原体的早期感染阶段主要依赖于脂肪酸降解来产生能量。这些数据提供了对辣椒疫霉生物学的更好理解，并提出了在两个不同发育阶段用于疾病控制的潜在代谢靶点。
The oomycete Phytophthora capsici is a plant pathogen responsible for important losses to vegetable production worldwide. Its asexual reproduction plays an important role in the rapid propagation and spread of the disease in the field. A global proteomics study was conducted to compare two key asexual life stages of P. capsici, i.e. the mycelium and cysts, to identify stage-specific biochemical processes. A total of 1200 proteins was identified using qualitative and quantitative proteomics. The transcript abundance of some of the enriched proteins was also analysed by quantitative real-time polymerase chain reaction. Seventy-three proteins exhibited different levels of abundance between the mycelium and cysts. The proteins enriched in the mycelium are mainly associated with glycolysis, the tricarboxylic acid (or citric acid) cycle and the pentose phosphate pathway, providing the energy required for the biosynthesis of cellular building blocks and hyphal growth. In contrast, the proteins that are predominant in cysts are essentially involved in fatty acid degradation, suggesting that the early infection stage of the pathogen relies primarily on fatty acid degradation for energy production. The data provide a better understanding of P. capsici biology and suggest potential metabolic targets at the two different developmental stages for disease control.