Effect of preincubation with ATP on ATP‐dependent reactions in sub‐mitochondrial particles
Effect of preincubation with ATP on ATP‐dependent reactions in sub‐mitochondrial particles
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ATP 预孵育对线粒体亚颗粒中 ATP 依赖性反应的影响
DOI：
10.1016/0014-5793(76)80828-9
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发表时间：
1976
期刊：
影响因子：
3.5
通讯作者：
D. Sanadi
中科院分区：
文献类型：
--
作者：
C. Kurup;D. Sanadi
在我们研究解偶联机制的过程中，我们观察到解偶联剂与线粒体和下软骨颗粒的结合受到 ATP 的显着抑制（未发表）。由于线粒体 ATP 酶复合物的蛋白质亚基似乎参与解偶联剂结合 [1, 2]，因此以能量相关反应为标准，研究 ATP 预孵育对膜供能的影响是很有意义的。这里给出的结果清楚地表明，用 ATP 进行预处理是 delt； e：与 ATP 依赖性膜供能有关，并且在与 ATP 预孵育期间，蛋白质从颗粒中释放出来。
During the course of our investigations on the mechanism of uncoupling, we observed that the binding of uncoupler to mitochondria and submitochondrial particles was considerably inhibited by ATP (unpublished). Since protein subunits of the mitochondrial ATPase complex seem to be involved in uncoupler binding [1, 2] it was of interest to study the effect of preincubation with ATP on membrane energization using energy-linked reactions as the criteria. The results presented here clearly indicate that pre-treatment with ATP is delt; e: ious to ATP-dependent membrane energization and that during preincubation with ATP, proteins are released from the particles.