Simultaneous implant placement and bone regeneration around dental implants using tissue-engineered bone with fibrin glue, mesenchymal stem cells and platelet-rich plasma
Simultaneous implant placement and bone regeneration around dental implants using tissue-engineered bone with fibrin glue, mesenchymal stem cells and platelet-rich plasma
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DOI：
10.1111/j.1600-0501.2006.01246.x
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发表时间：
2006-10-01
影响因子：
4.3
通讯作者：
Ueda, Minoru
中科院分区：
文献类型：
--
作者：
Ito, Kenji;Yamada, Yoichi;Ueda, Minoru
本研究旨在评价组织工程骨作为植骨材料用于牙槽嵴增高同时种植体植入的效果。本研究中使用了12只成年杂种犬。在下颌骨区域拔牙后1个月，使用直径为10 mm的环钻棒在下颌骨两侧诱导骨缺损。通过髂骨活检获得犬间充质干细胞（dMSCs），并在植入前培养4周。在安装牙种植体后，缺损处同时植入以下移植材料：（i）纤维蛋白，（ii）dMSC和纤维蛋白（dMSC/纤维蛋白），（iii）dMSC、富血小板血浆（PRP）和纤维蛋白（dMSC/PRP/纤维蛋白）和（iv）对照（仅缺损处）。植入后2、4和8周，通过组织学和组织形态计量学分析评估植入物。种植体表现出不同程度的骨-种植体接触（BIC）。2、4和8周后的BIC分别为17%、19%和29%（对照），20%、22%和25%（纤维蛋白），22%、32%和42%（dMSC/纤维蛋白）以及25%、49%和53%（dMSC/PRP/纤维蛋白）。这项研究表明，组织工程骨可能是足够的质量，可预测的增强骨再生周围的牙种植体时，同时使用与种植体放置。
This study was undertaken to evaluate the use of tissue-engineered bone as grafting material for alveolar augmentation with simultaneous implant placement. Twelve adult hybrid dogs were used in this study. One month after the extraction of teeth in the mandible region, bone defects on both sides of the mandible were induced using a trephine bar with a diameter of 10 mm. Dog mesenchymal stem cells (dMSCs) were obtained via iliac bone biopsy and cultured for 4 weeks before implantation. After installing the dental implants, the defects were simultaneously implanted with the following graft materials: (i) fibrin, (ii) dMSCs and fibrin (dMSCs/fibrin), (iii) dMSCs, platelet-rich plasma (PRP) and fibrin (dMSCs/PRP/fibrin) and (iv) control (defect only). The implants were assessed by histological and histomorphometric analysis, 2, 4 and 8 weeks after implantation. The implants exhibited varying degrees of bone-implant contact (BIC). The BIC was 17%, 19% and 29% (control), 20%, 22% and 25% (fibrin), 22%, 32% and 42% (dMSCs/fibrin) and 25%, 49% and 53% (dMSCs/PRP/fibrin) after 2, 4 and 8 weeks, respectively. This study suggests that tissue-engineered bone may be of sufficient quality for predictable enhancement of bone regeneration around dental implants when used simultaneous by with implant placement.