A potential genetic control by suppression of the wing developmental gene wingless in a global invasive pest Bactrocera dorsalis
A potential genetic control by suppression of the wing developmental gene wingless in a global invasive pest Bactrocera dorsalis
复制标题
通过抑制全球入侵害虫橘小实蝇翅膀发育基因的潜在遗传控制
DOI：
10.1007/s10340-020-01263-1
复制
发表时间：
2020-07-18
影响因子：
4.8
通讯作者：
Li, Zhihong
中科院分区：
文献类型：
--
作者：
Guo, Shaokun;Guo, Xiaoyu;Li, Zhihong
入侵有害生物对生态系统的不利影响逐渐加剧，入侵有害生物的防治是长期以来的研究热点。基因控制策略是一种有效、可持续和环境友好的控制害虫的方法，在世界范围内受到了极大的关注。然而，桔小实蝇是一种飞行能力较强的全球性入侵害虫，对其遗传控制的研究较少。B区的侧翼。背部作为重要的飞行器官，可能是其广泛发生的主要原因。在这里，我们首先分析了B的机翼结构。背飞虱发现其翅膀具有典型的双翅目昆虫翅膀特征。采用实时荧光定量聚合酶链式反应、抗体染色和RNA原位杂交等方法检测翅膀发育相关基因的时空表达。通过RNA干扰从9个基因中筛选出影响翅发育的关键基因WG，并将其用于田间防治。多萨利斯。模拟对照后，子代数量和二化螟成虫率均显著下降。本研究为通过调控翅发育基因进行害虫遗传控制的应用提供了初步支持，为解决褐飞虱的广泛传播问题提供了新的思路。多萨利斯。
The adverse effects of invasive pests on ecosystems have gradually intensified, and the prevention of invasive pests is a long-standing research focus. Genetic control strategies are effective, sustainable and environmentally friendly methods for controlling pests and have received substantial attention worldwide. However, there is a lack of researches on the genetic control ofBactrocera dorsalisHendel, a global invasive pest with strong flight ability. The wing ofB. dorsalisas an important flight organ may be a main reason for its widespread occurrence. Here, we first analysed the wing structure ofB. dorsalisand found that its wing has the typical characteristic of Diptera insect wing. The spatiotemporal expressions of the wing development genes were examined by quantitative real-time PCR, antibody staining and RNA in situ hybridization. The results indicated that the wing development genes were significantly upregulated in the pupal stage, and the regional expression of each gene was clarified.Wingless(wg), a key gene that significantly affects wing development, was selected from nine genes through RNA interference and used to simulate the field control ofB. dorsalis. The offspring population and the fruit-borer rate decreased significantly after the simulated control. This study provides preliminary support for the application of genetic pest control by regulating the wing development gene and proposes a novel idea for solving the problem of the extensive spread ofB. dorsalis.