REGULATION OF THE COMPLEX PATTERN OF SEVENLESS EXPRESSION IN THE DEVELOPING DROSOPHILA EYE
REGULATION OF THE COMPLEX PATTERN OF SEVENLESS EXPRESSION IN THE DEVELOPING DROSOPHILA EYE
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DOI：
10.1073/pnas.86.16.6245
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
RUBIN, GM
中科院分区：
文献类型：
--
作者：
BOWTELL, DDL;KIMMEL, BE;RUBIN, GM
Seven less基因编码一种具有蛋白酪氨酸激酶活性的细胞表面受体，在发育中的果蝇眼睛中以高度特异和复杂的模式表达。我们已经将SEVENLESS启动子与受体基因LacZ偶联，并检测了转基因幼虫发育过程中β-半乳糖苷酶的表达模式。我们的结果表明，Seven less蛋白的动态表达模式是受转录调控的。位于编码区5‘’的启动子序列不足以满足基因表达的野生型水平，但似乎能够赋予正确的表达模式。相反，基因体内的增强子序列可以在Seven less启动子或异源启动子上提供正确的模式和正常水平的表达。因此，Seven less表达的复杂模式被冗余地编码在基因近端的启动子序列和基因内部的增强子元件中，但依赖于这些增强子序列进行正确的定量表达。
The sevenless gene encodes a cell surface receptor that has protein-tyrosine kinase activity and is expressed in a highly specific and complex pattern in the developing Drosophila eye. We have coupled the sevenless promoter to the receptor gene lacZ and have examined the pattern of .beta.-galactosidase expression in the developing eyes of transgenic larvae. Our results indicate that the dynamic pattern of sevenless protein expression is regulated transcriptionally. Promoter sequences located 5'' of the coding region are insufficient for the wild-type level of gene expression but appear to be able to confer the correct pattern of expression. In contrast, enhancer sequences within the body of the gene can confer both the correct pattern and a normal level of expression on either the sevenless promoter or heterologous promoters. Thus the complex pattern of sevenless expression is redundantly encoded within proximal promoter sequences and enhancer elements internal to the gene but reflies on these enhancer sequences for correct quantitative expression.