Rapid and versatile construction of diverse and functional nanostructures derived from a polyphosphoester-based biomimetic block copolymer system.
Rapid and versatile construction of diverse and functional nanostructures derived from a polyphosphoester-based biomimetic block copolymer system.
复制标题
DOI：
10.1021/ja309037m
复制
发表时间：
2012-11-07
影响因子：
15
通讯作者：
Wooley, Karen L.
中科院分区：
文献类型：
--
作者：
Zhang, Shiyi;Zou, Jiong;Zhang, Fuwu;Elsabahy, Mahmoud;Felder, Simcha E.;Zhu, Jiahua;Pochan, Darrin J.;Wooley, Karen L.
已经开发了一种用于制备和修饰多功能聚合物纳米颗粒的快速有效的方法，对于该方法，从小分子到聚合物再到纳米颗粒的整个工程过程绕过了典型的缓慢和低效的程序，而是采用一系列步骤来完全捕捉“点击”在过去的十年里，化学概念极大地促进了复杂聚合物材料的制备。从多功能平台构建具有功能复杂性的各种纳米颗粒是一个具有挑战性的目标，为基础研究提供材料，并优化各种应用。在本文中，我们展示了一个家庭的纳米粒子具有不同的表面电荷和功能的基础上，可生物降解的聚磷酸酯嵌段共聚物系统的快速和简便的制备。从逆合成的角度来看，非离子，阴离子，阳离子和两性离子胶束的流体动力学直径之间的13 nm至21 nm和大的尺寸均匀性，快速形成悬浮，独立的，四个两亲性二嵌段聚磷酸酯到水中，这是功能化从相同的亲本疏水功能AB二嵌段聚磷酸酯通过“点击”型硫醇-炔反应。通过有机催化超快速开环聚合（< 5 min），采用两步一锅法合成了具有良好疏水功能性（PDI < 1.2）的AB二嵌段聚磷酸酯。从小分子到纳米颗粒的整个可编程过程可以在6 h内完成，作为阴离子和非离子纳米颗粒的最快速方法，尽管阳离子和两性离子纳米颗粒需要约10 min。2天，由于透析纯化。胶束显示出高的生物相容性，与Lipofectamine®商业转染剂相比，即使是阳离子胶束也表现出对RAW 264.7小鼠巨噬细胞低6倍的细胞毒性。
A rapid and efficient approach for the preparation and modification of a versatile class of functional polymer nanoparticles has been developed, for which the entire engineering process from small molecules to polymers to nanoparticles bypasses typical slow and inefficient procedures, and rather employs a series of steps that capture fully the “click” chemistry concepts that have greatly facilitated the preparation of complex polymer materials over the past decade. The construction of various nanoparticles with functional complexity from a versatile platform is a challenging aim to provide materials for fundamental studies and also optimization toward a diverse range of applications. In this paper, we demonstrate the rapid and facile preparation of a family of nanoparticles with different surface charges and functionalities based on a biodegradable polyphosphoester block copolymer system. From a retrosynthetic point of view, the non-ionic, anionic, cationic and zwitterionic micelles with hydrodynamic diameters between 13 nm to 21 nm and great size uniformity were quickly formed by suspending, independently, four amphiphilic diblock polyphosphoesters into water, which were functionalized from the same parental hydrophobic-functional AB diblock polyphosphoester by “click” type thiol-yne reactions. The well-defined (PDI < 1.2) hydrophobic-functional AB diblock polyphosphoester was synthesized by an ultrafast (< 5 min) organocatalyzed ring-opening polymerization in a two-step, one-pot manner with the quantitative conversions of two kinds of cyclic phospholane monomers. The whole programmable process starting from small molecules to nanoparticles could be completed within 6 h, as the most rapid approach for the anionic and non-ionic nanoparticles, although the cationic and zwitterionic nanoparticles required ca. 2 days due to purification by dialysis. The micelles showed high biocompatibility, with even the cationic micelles exhibiting a 6-fold lower cytotoxicity toward RAW 264.7 mouse macrophage cells, as compared to the Lipofectamine® commercial transfection agent.
登录
查看更多内容
影响因子：
4.6
作者：
Shao, Haiyan;Zhang, Mingzu;Ni, Peihong
通讯作者：
Ni, Peihong
影响因子：
5.5
作者：
Iwasaki, Yasuhiko;Wachiralarpphaithoon, Chookaet;Akiyoshi, Kazunari
通讯作者：
Akiyoshi, Kazunari
影响因子：
46.2
作者：
Elsabahy M;Wooley KL
通讯作者：
Wooley KL
均聚物的分子自组装：制造癌症治疗药物输送平台的替代方案
影响因子：
16.6
作者：
Liu, Jinyao;Huang, Wei;Yan, Deyue
通讯作者：
Yan, Deyue
用于高效抗癌药物输送的定制双 pH 敏感聚合物-阿霉素纳米颗粒
影响因子：
15
作者：
Du, Jin-Zhi;Du, Xiao-Jiao;Wang, Jun
通讯作者：
Wang, Jun