Ethylene receptor degradation controls the timing of ripening in tomato fruit
Ethylene receptor degradation controls the timing of ripening in tomato fruit
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DOI：
10.1111/j.1365-313x.2007.03170.x
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发表时间：
2007-08-01
期刊：
影响因子：
7.2
通讯作者：
Klee, Harry J.
中科院分区：
文献类型：
--
作者：
Kevany, Brian M.;Tieman, Denise M.;Klee, Harry J.
番茄果实的成熟需要发育线索和植物激素乙烯的协调。多基因乙烯受体家族已被证明可以负向调节乙烯信号转导并抑制乙烯反应。在这里，我们证明两个家族成员LeETR4或LeETR6的水平降低会导致早熟表型。我们提供的证据表明，受体在乙烯存在的情况下会迅速降解，并且降解可能通过 26S 蛋白酶体依赖性途径发生。未成熟果实暴露于乙烯会导致受体蛋白量减少和提前成熟。结果与受体水平通过测量累积乙烯暴露来调节果实成熟开始时间的模型一致。
Fruit ripening in tomato requires the coordination of both developmental cues and the phytohormone ethylene. The multigene ethylene receptor family has been shown to negatively regulate ethylene signal transduction and suppress ethylene responses. Here we demonstrate that reduction in the levels of either of two family members, LeETR4 or LeETR6, causes an early-ripening phenotype. We provide evidence that the receptors are rapidly degraded in the presence of ethylene, and that degradation probably occurs through the 26S proteasome-dependent pathway. Ethylene exposure of immature fruits causes a reduction in the amount of receptor protein and earlier ripening. The results are consistent with a model in which receptor levels modulate timing of the onset of fruit ripening by measuring cumulative ethylene exposure.