Determination of DL-tetrahydropalmatine in Corydalis yanhusuo by L-tetrahydropalmatine imprinted monolithic column coupling with reversed-phase high performance liquid chromatography
Determination of DL-tetrahydropalmatine in Corydalis yanhusuo by L-tetrahydropalmatine imprinted monolithic column coupling with reversed-phase high performance liquid chromatography
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DOI：
10.1016/j.chroma.2006.03.084
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发表时间：
2006-06-09
影响因子：
4.1
通讯作者：
Zou, Hanfa
中科院分区：
文献类型：
--
作者：
Ou, Junjie;Kong, Liang;Zou, Hanfa
建立了L-THP印迹整体柱前在线/离线耦合反相高效液相色谱（RP-HPLC）直接测定中药延胡索中dl -四氢巴马汀（DL-THP）的方法。以甲基丙烯酸（MAA）和二甲基丙烯酸乙酯（EDMA）为功能单体和交联剂，采用原位聚合法制备了L-THP印迹整体柱。通过对流动相组成、流速、柱温、进样量等对映体分离条件的优化，在MIP上对DL-THP进行了基线分离。采用压印整体柱作为预柱对延胡索提取物进行分馏。采用反相高效液相色谱法分离非保留部分和保留部分。同时，在未保留部分和保留部分中分别检测到D-THP和L-THP。此外，利用分子印迹单体在线耦合反相柱直接测定L-THP。(c) 2006 Elsevier B.V.版权所有
A method for direct determination of DL-tetrahydropalmatine (DL-THP) in Corydalis yanhusuo, a traditional Chinese herb, by L-THP imprinted monolithic precolumn on-line/off-line coupling with reversed-phase high performance liquid chromatography (RP-HPLC) was developed. The L-THP imprinted monolithic column has been prepared by in situ polymerization using methacrylic acid (MAA) and ethylene dimethacrylate (EDMA) as functional monomer and cross-linker, respectively. With the optimization of chromatographic conditions, such as mobile phase composition, flow rate, column temperature and sample loading, for the separation of enantiomer, DL-THP was base-line separated on the MIP. The imprinted monolithic column was used as a precolumn for fractionation of the C. yanhusuo extract. Both the non-retained and retained fractions were separated by RP-HPLC. Meanwhile, the D-THP and L-THP can be detected in the non-retained and retained fractions, respectively. Additionally, direct determination of L-THP using molecularly imprinted monolith on-line coupling with a reversed-phase column was acquired. (c) 2006 Elsevier B.V. All rights reserved.