Comparable endotoxic properties of lipopolysaccharides are manifest in diverse clinical isolates of gram-negative bacteria
Comparable endotoxic properties of lipopolysaccharides are manifest in diverse clinical isolates of gram-negative bacteria
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DOI：
10.1128/iai.68.4.1899-1904.2000
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发表时间：
2000-04-01
影响因子：
3.1
通讯作者：
Morrison, DC
中科院分区：
文献类型：
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作者：
Luchi, M;Morrison, DC
一般来说，脓毒症患者血清脂多糖(LPS；内毒素的生物活性成分)水平与死亡率之间的相关性很差。这项研究的目的是确定来自不同临床分离的革兰氏阴性细菌的脂多糖之间的化学、结构或生物学差异是否可以解释这种差异。采用热酚-水抽提法从8株革兰氏阴性杆菌临床分离株中制备脂多糖。磷酸盐含量为3.0-13.8%(平均6.7+/-3.6%)，2-酮-3-脱氧辛糖酸盐含量为1.9-27.4%(平均8.9+/-8.5%)。这些值与参考内毒素的结果没有什么不同。在内毒素活性的标准测量中，最低活性的制剂和最高活性的制剂之间存在大约两倍的差异。这两种制剂在诱导小鼠腹膜巨噬细胞分泌肿瘤坏死因子α的能力方面差异最大，但在D(+)-氨基半乳糖致敏小鼠的致死率上相似。这些相对较小的内毒素活性差异似乎不太可能解释通常观察到的革兰氏阴性脓毒症患者血清内毒素水平与死亡率之间的差异。
In general there is a poor correlation between serum lipopolysaccharide (LPS; the biologically active constituent of endotoxin) levels and mortality in septic patients. The objective of this study was to determine if chemical, structural, or biological differences among LPS from different clinical isolates of gram-negative bacteria might explain this discrepancy. LPS preparations were made using the hot phenol-water extraction method from eight clinical isolates of gram-negative bacteria. As a percentage of the total weight of the LPS, the phosphate content ranged from 3.0 to 13.8% (average, 6.7 +/- 3.6%), and the 2-keto-3-deoxyoctonate content ranged from 1.9 to 27.4% (average, 8.9 +/- 8.5%). These values were not dissimilar to those obtained for a reference endotoxin. In a standard measure of LPS activity, the Limulus amoebocyte lysate assay, there was approximately a twofold difference between the least and most active preparations. The two preparations,vith the greatest difference in their ability to elicit the secretion of tumor necrosis factor alpha from a mouse peritoneal macrophage cell line were similar in lethality when administered to mice sensitized to the effects of LPS by D(+)-galactosamine. These relatively minor differences in LPS activity seem unlikely to explain the generally observed discrepancy between serum endotoxin levels and mortality in patients with gram-negative sepsis.