Effects of ginseng saponins isolated from red ginseng on ultraviolet B-induced skin aging in hairless mice
Effects of ginseng saponins isolated from red ginseng on ultraviolet B-induced skin aging in hairless mice
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DOI：
10.1016/j.ejphar.2008.11.021
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发表时间：
2009-01-05
影响因子：
5
通讯作者：
Kimura, Yoshiyuki
中科院分区：
文献类型：
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作者：
Kim, Young Gon;Sumiyoshi, Maho;Kimura, Yoshiyuki
众所周知，低剂量的慢性紫外线B（UVB）暴露导致皮肤光老化，包括皮肤厚度和皱纹形成的增加以及皮肤弹性的降低。本研究检测了从红参根中分离的总皂苷和抗氧化剂Rb-1对无毛小鼠皮肤厚度、弹性和皱纹形成的影响，这些皮肤由长期低剂量UVB照射引起。局部应用人参总皂苷（10 μ g或100 ng/小鼠）和抗Rb-1（100 fg，10 μ g或1 ng/小鼠）显着抑制皮肤厚度的增加和皱纹的形成和减少皮肤弹性诱导的长期UVB照射。此外，我们还研究了总皂苷和UVB照射的无毛小鼠皮肤中的Rb-1的组织学效应。UVB照射诱导的凋亡、Ki-67和8-羟基-2 '-脱氧鸟苷阳性细胞的增加可通过局部应用总皂苷和抗凋亡Rb-1来预防。此外，人参总皂苷和人参皂苷Rb-1可防止长时间UVB照射引起的胶原纤维破坏。人参皂苷Rb-1（100 fg，10 pg，1 ng/ml）增加UVB处理的人角质形成细胞中Bcl-2的表达水平。Rb-1可能通过上调Bcl-2的表达而对UVB诱导的细胞凋亡起保护作用。这些结果表明，抗紫外线Rb-1对皮肤光老化的保护作用可能是由于增加胶原合成和/或抑制基质金属蛋白酶的表达在真皮成纤维细胞。(C)2008 Elsevier B. V.保留所有权利。
It is well-known that chronic ultraviolet B (UVB) exposure at low-dose causes skin photoaging including increases in skin thickness and wrinkle formation and reduction in skin elasticity. This study examined the effects of total saponins and ginsenoside Rb-1 isolated from Red Ginseng roots on skin thickness, elasticity, and wrinkle formation caused by long-term, low-dose UVB irradiation in hairless mice. The topical application of total ginseng saponins (10 pg or 100 ng/mouse) and ginsenoside Rb-1 (100 fg, 10 pg, or 1 ng/mouse) significantly inhibited increases in skin thickness and wrinkle formation and the reduction in skin elasticity induced by long-term UVB irradiation. Furthermore, we examined the histological effects of total saponins and ginsenoside Rb-1 in the skin of UVB-irradiated hairless mice. The increases in apoptotic, Ki-67-, and 8-hydroxy-2'-deoxyguanosine-positive cells induced by UVB exposure were prevented by the topical application of total saponins and ginsenoside Rb-1. Furthermore, total saponins and ginsenoside Rb-1 prevented the disruption of collagen fibers induced by the long-term UVB irradiation. Ginsenoside Rb-1 (100 fg, 10 pg, and 1 ng/ml) increased the Bcl-2 expression level in UVB-treated human keratinocytes. The protective effect of ginsenoside Rb-1 on UVB-mediated apoptosis may be due to the up-regulation of Bcl-2 expression. These results suggest that the protective effect of ginsenoside Rb-1 on skin photoaging induced bychronic UVB exposure may be due to the increase in collagen synthesis and/or the inhibition of matrix metalloproteinase expression in dermal fibroblasts. (C) 2008 Elsevier B.V. All rights reserved.