Methodological considerations for improving Western blot analysis
Methodological considerations for improving Western blot analysis
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DOI：
10.1016/j.vascn.2009.12.001
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发表时间：
2010-03-01
影响因子：
1.9
通讯作者：
MacPhee, Daniel J.
中科院分区：
文献类型：
--
作者：
MacPhee, Daniel J.
对一种能够测定抗血清抗原特异性的技术的需求导致了一种方法的发展，这种方法可以将在聚丙烯酰胺凝胶上电泳分离的蛋白质复制到硝化纤维素膜上。这种方法被称为Western blotting，对于研究特定蛋白的存在、相对丰度、相对分子质量、翻译后修饰和相互作用非常有用。因此，它在化学、生物学和生物医学等许多科学研究领域中被常规使用。这篇综述旨在触及一些应该考虑的方法条件，以改进Western blot分析，特别是作为研究生的指导，以及希望继续采用这一基础研究工具的科学家。(C) 2009爱思唯尔公司版权所有。
The need for a technique that could allow the determination of antigen specificity of antisera led to the development of a method that allowed the production of a replica of proteins, which had been separated electrophoretically on polyacrylamide gels, on to a nitrocellulose membrane. This method was coined Western blotting and is very useful to study the presence, relative abundance, relative molecular mass, post-translational modification, and interaction of specific proteins. As a result it is utilized routinely in many fields of scientific research such as chemistry, biology and biomedical sciences. This review serves to touch on some of the methodological conditions that should be considered to improve Western blot analysis, particularly as a guide for graduate students but also scientists who wish to continue adapting this now fundamental research tool. (C) 2009 Elsevier Inc. All rights reserved.