The engineering of doxorubicin-loaded liposome-quantum dot hybrids for cancer theranostics
The engineering of doxorubicin-loaded liposome-quantum dot hybrids for cancer theranostics
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DOI：
10.1088/1674-1056/23/8/087805
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发表时间：
2014-06
影响因子：
1.7
通讯作者：
Bowen Tian;W. Al-Jamal;Kostas Kostarelos
中科院分区：
文献类型：
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作者：
Bowen Tian;W. Al-Jamal;Kostas Kostarelos
最近，许多研究试图通过将量子点（QD）的上级荧光性质与治疗能力整合到用于癌症治疗诊断的单个囊泡中来开发多功能纳米结构。脂质体-量子点（L-QD）杂化囊泡在构建用于癌症成像和治疗的多功能纳米结构方面显示出了很大的潜力。为了实现这样的潜力，我们在这里报告的进一步功能化的L-QD混合囊泡的治疗能力，通过装载抗癌药物阿霉素（Dox）到其水核心。首先通过将TOPO封端的CdSe/ZnS QD掺入DSPC：Chol：DSPE-PEG 2000的脂质双层中，然后使用pH梯度技术加载Dox来工程化L-QD杂化囊泡。Dox在L-QD混合囊泡中的装载效率达到高达97%，与脂质体对照相当。所有这些证据证明，QD掺入到脂质双层中不影响使用pH梯度技术通过脂质体的脂质膜的Dox负载。此外，释放研究表明，Dox释放曲线可以简单地通过改变脂质组成来调节。总之，这里提出的装载Dox的L-QD混合囊泡构成了一种有前途的多功能纳米结构，能够运输治疗和诊断方式的组合。
Many studies have recently attempted to develop multifunctional nanoconstructs by integrating the superior fluorescence properties of quantum dots (QD) with therapeutic capabilities into a single vesicle for cancer theranostics. Liposome-quantum dot (L-QD) hybrid vesicles have shown promising potential for the construction of multifunctional nanoconstructs for cancer imaging and therapy. To fulfil such a potential, we report here the further functionalization of L-QD hybrid vesicles with therapeutic capabilities by loading anticancer drug doxorubicin (Dox) into their aqueous core. L-QD hybrid vesicles are first engineered by the incorporation of TOPO-capped, CdSe/ZnS QD into the lipid bilayers of DSPC:Chol:DSPE-PEG2000, followed by Dox loading using the pH-gradient technique. The loading efficiency of Dox into L-QD hybrid vesicles is achieved up to 97%, comparable to liposome control. All these evidences prove that the incorporation of QD into the lipid bilayer does not affect Dox loading through the lipid membrane of liposomes using the pH-gradient technique. Moreover, the release study shows that Dox release profile can be modulated simply by changing lipid composition. In conclusion, the Dox-loaded L-QD hybrid vesicles presented here constitute a promising multifunctional nanoconstruct capable of transporting combinations of therapeutic and diagnostic modalities.