Identification of marine traditional Chinese medicine dried seahorses in the traditional Chinese medicine market using DNA barcoding
Identification of marine traditional Chinese medicine dried seahorses in the traditional Chinese medicine market using DNA barcoding
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DOI：
10.1080/24701394.2016.1248430
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发表时间：
2018-01-01
影响因子：
--
通讯作者：
Guo, Jinlin
中科院分区：
文献类型：
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作者：
Hou, Feixia;Wen, Longlian;Guo, Jinlin
中国药典记载的海马具有重要的药用功效，并被用作传统中药的成分。日益增长的国际贸易威胁着这个物种。DNA条形码技术在野生动物保护中具有巨大的应用潜力，可以有效地防止濒危物种的非法贸易。利用COI基因对海马进行了鉴定，在我国三大中药市场发现了9种海马。所有种间遗传变异均大于2%。物种间的平均遗传距离比物种内的平均遗传距离大17倍。系统发育树显示，各物种聚在相应的分支。结果表明，COI条形码技术可以用于海马种类的识别，并在海马贸易监测中发挥重要作用。
Seahorse documented in Chinese pharmacopeia possess important medicinal efficacy and are used as an ingredient in traditional Chinese medicines. The growing international trade threatens the species. DNA barcoding holds a great application potentiality in wildlife conservation and might prevent the illegal trade of threatened species. The COI gene was used to identify seahorse, and nine Hippocampus species were found in the three large traditional Chinese medicines markets of China. All inter-specific genetic variations were larger than 2%. Mean genetic distances between species were 17-fold larger than those within the species. Phylogenetic tree showed that each species clustered in the appropriate branch. All results demonstrated that COI-based barcoding technique could be used to identify seahorse species and played a major role in monitoring the seahorse trade.