NUMBER AND CONTINUOUS PROLIFERATIVE PATTERN OF TRANSPLANTED PRIMITIVE IMMUNOHEMATOPOIETIC STEM-CELLS
NUMBER AND CONTINUOUS PROLIFERATIVE PATTERN OF TRANSPLANTED PRIMITIVE IMMUNOHEMATOPOIETIC STEM-CELLS
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DOI：
10.1073/pnas.85.3.822
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发表时间：
1988-02-01
影响因子：
11.1
通讯作者：
LERNER, C
中科院分区：
文献类型：
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作者：
HARRISON, DE;ASTLE, CM;LERNER, C
我们估计了可移植原始干细胞 (PSC) 的数量，并发现证据表明同一 PSC 持续产生循环红细胞和淋巴细胞。这些估计使用二项式公式计算来自含有两种可区分细胞类型的相同部分骨髓混合物的接受者的数据。方差分析用于比较每个接受者内的重复测试。还估计了 .pi.s 或 .pi.c 的值，即两个独立采样的细胞从同一 PSC 分离的概率，因为这不需要所有 PSC 对分化细胞群的贡献相同的未经验证的条件。移植后几个月，每1-2次仅从单个PSC衍生出红细胞。注射了 105 个骨髓细胞，比之前报道的少数倍。在接受2-8次的组中，每个接受者的红细胞和淋巴细胞类型的百分比密切相关，r值范围为0.86至0.94。 105个骨髓细胞；显然，相同的前体细胞在每个受体中重新填充了骨髓系和淋巴系，正如对真正的 PSC 所预期的那样。我们的数据不符合克隆继承模型，该模型预测新 PSC 的连续激活和旧 PSC 的失活。在 76 至 154 天之间，分化的红细胞前体可能耗尽，没有证据表明新的前体激活或在 154 至 250 天之间进一步变化。当移植后165至295天对各个受者进行随访时，每种供体类型新产生的红细胞（网织红细胞）的百分比几乎没有变化，并且在8至200倍的骨髓剂量下受者内的差异相似。 105细胞，进一步与PSC克隆的顺序激活和失活模型相矛盾。因此，移植的 PSC 在接受者一生的大部分时间里都持续活跃。
We estimated numbers of transplantable primitive stem cells (PSCs) and found evidence that the same PSC continuously produced circulating erythrocytes and lymphocytes. These estimates used the binomial formula on data from recipients of identical portions of marrow mixtures containing two distinguishable cell types. Analysis of variance was used to compare repeated tests within each recipient. Values of .pi.s or .pi.c, probabilites that two independently sampled cells were descenced from the same PSC, were also estimated, as this does not require the unverified condition that all PSCs contribute equally to the differentiated cell population. Several months after transplantation, erythrocytes were descended from only a single PSC per 1-2 .times. 105 marrow cells injected, several times rarer than previously reported. Percentages of erythrocyte and lymphocyte types in each recipient were closely correlated, with r values ranging from 0.86 to 0.94, in groups receivig 2-8 .times. 105 marrow cells; apparently the same precursors repopulated both myeloid and lymphoid lines in each recipient, as expected of true PSCs. Our data did not fit the clonal succession model, which predicts sequential activation of new PSCs and deactivation of old. Between 76 and 154 days, differentiated erythrocyte precursors were probably exhausted, with no evidence for new precursor activation or for further change between 154 and 250 days. The percentage of newly produced erythrocytes (reticulocytes) of each donor type varied little when individual recipients were followed between 165 and 295 days after transplantation, and variances within recipients were similar at marrow doses from 8 to 200 .times. 105 cells, further contradicting models of sequential activation and deactivation of PSC clones. Thus, transplanted PSCs were continually active during much of the recipient''s lifespan.