Nano-Structured Gelatin/Bioactive Glass Hybrid Scaffolds for the Enhancement of Odontogenic Differentiation of Human Dental Pulp Stem Cells.
Nano-Structured Gelatin/Bioactive Glass Hybrid Scaffolds for the Enhancement of Odontogenic Differentiation of Human Dental Pulp Stem Cells.
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DOI：
10.1039/c3tb21002b
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发表时间：
2013-10-07
期刊：
影响因子：
--
通讯作者：
Liu X
中科院分区：
文献类型：
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作者：
Qu T;Liu X
蛀牙是全世界最常见的慢性疾病之一。再生腐烂的牙本质/牙髓结构需要设计新型支架材料，模拟天然牙细胞外基质（ECM）的结构，并为牙髓干细胞（DPSC）的附着、增殖、分化和生物矿化提供合适的环境。在这项工作中，我们开发了一种制备三维（3D）纳米纤维明胶/二氧化硅生物活性玻璃（NF-明胶/SBG）混合支架的方法，该支架模仿天然牙科ECM的纳米结构结构和化学成分。该方法结合了热诱导相分离、溶胶-凝胶和致孔剂浸出过程，合成了具有天然 ECM 结构、高孔隙率、明确的孔径和互连性以及更高的机械强度的混合支架。体外细胞培养研究表明，在相同条件下，与 NF-明胶支架相比，人 DPSC 在 NF-明胶/SBG 支架上的增殖率显着更高。此外，SBG 整合到混合支架中显着促进了人类 DPSC 的分化和生物矿化。 NF-明胶/SBG组的碱性磷酸酶（ALP）活性和牙源分化标记基因（Col I、ALP、OCN、DSPP和DMP-1）的表达均显着高于NF-明胶组。这些结果通过苏木精和伊红 (H&E) 和 von Kossa 染色得到进一步证实，混合支架中 ECM 分泌和矿物质沉积量增加证明了这一点。总之，仿生NF-明胶/SBG混合支架为人类DPSC的生长和分化提供了良好的环境，是牙本质/牙髓组织再生的有希望的候选者。
Tooth decay is one of the most common chronic disorders throughout the world. Regenerating decayed dentin/pulp structure requires the design of novel scaffolding materials that mimic the architecture of natural dental extracellular matrix (ECM) and provide suitable environments for the attachment, proliferation, differentiation, and biomineralization of dental pulp stem cells (DPSCs). In this work, we developed an approach to prepare three-dimensional (3D) nano-fibrous gelatin/silica bioactive glass (NF-gelatin/SBG) hybrid scaffolds that mimic the nano-structured architecture and chemical composition of natural dental ECM. This approach involved the combination of a thermally induced phase separation, sol-gel, and porogen leaching process, and synthesized hybrid scaffolds possessing natural ECM-like architecture, high porosity, well-defined pore size and interconnectivity, and improved mechanical strength. An in vitro cell culture study showed that human DPSCs had a significantly higher proliferation rate on NF-gelatin/SBG scaffolds compared to NF-gelatin scaffolds under the same conditions. Furthermore, the integration of SBG into the hybrid scaffold significantly promoted the differentiation and biomineralization of the human DPSCs. The alkaline phosphatase (ALP) activity and expressions of marker genes for odontogenic differentiation (Col I, ALP, OCN, DSPP and DMP-1) were all significantly higher in the NF-gelatin/SBG than in the NF-gelatin group. Those results were further confirmed by hematoxylin and eosin (H&E) and von Kossa staining, as evidenced by greater ECM secretion and mineral deposition in the hybrid scaffold. In summary, the biomimetic NF-gelatin/SBG hybrid scaffolds provide an excellent environment for the growth and differentiation of human DPSCs and are promising candidates for dentin/pulp tissue regeneration.