Selection and characterization of novel DNA aptamer against colorectal carcinoma Caco-2 cells.
Selection and characterization of novel DNA aptamer against colorectal carcinoma Caco-2 cells.
复制标题
针对结直肠癌 Caco-2 细胞的新型 DNA 适体的选择和表征。
DOI：
10.1002/bab.1737
复制
发表时间：
2019
影响因子：
2.8
通讯作者：
那仁满都拉
中科院分区：
文献类型：
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作者：
Maimaitiyiming Y;Yang C;Wang Y;Hussain L;那仁满都拉
适体是短的单链核酸（DNA或RNA）寡核苷酸，可以通过体外指数富集配体系统进化（SELEX）技术获得。由于其上级性质，如小尺寸、高结合亲和力和稳定性，它们被认为是用于疾病诊断和治疗的可行工具。在本研究中，我们试图通过基于细胞的SELEX方法筛选高亲和力的DNA适体，以选择性地靶向结直肠癌Caco-2细胞。在连续14轮选择后，鉴定了适体ApC 1。共聚焦显微镜结果显示，ApC 1可以快速内化到Caco‐2细胞中，但不能内化到HEK 293细胞中。此外，它对Caco-2细胞而不是其他细胞系（如293 T、HeLa、MCF-7、HL-60和NB 4）显示出高特异性。综上所述，我们的研究结果表明ApC 1适体对结直肠癌Caco-2细胞具有高度的特异性，可以在未来的研究中进一步应用于结直肠癌的靶向治疗。
Aptamers are short, single‐stranded nucleic acid (DNA or RNA) oligonucleotides that can be obtained by a technique called systematic evolution of ligands by exponential enrichment (SELEX)in vitro. Due to superior properties such as small size, high binding affinity, and stability, they are considered to be feasible tools for diagnosis and treatment of disease. In the current study, we attempted to screen a high‐affinity DNA aptamer to selectively target the colorectal carcinoma Caco‐2 cells by using cell‐based SELEX approach. After 14 consecutive rounds of selection, aptamer ApC1 was identified. Confocal microscopy results revealed that ApC1 could rapidly internalize into Caco‐2 cells but not HEK 293 cells. Moreover, it showed high specificity to Caco‐2 cells rather than other cell lines such as 293T, HeLa, MCF‐7, HL‐60, and NB4. Collectively, our results demonstrated that aptamer ApC1 has high specificity to colorectal carcinoma Caco‐2 cells, which could be further applied for targeted therapy of colorectal cancer in future studies.