Cell interaction with three-dimensional sharp-tip nanotopography
Cell interaction with three-dimensional sharp-tip nanotopography
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DOI：
10.1016/j.biomaterials.2006.11.031
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发表时间：
2007-03-01
期刊：
影响因子：
14
通讯作者：
Kim, Chang-Jin
中科院分区：
文献类型：
--
作者：
Choi, Chang-Hwan;Hagvall, Sepideh H.;Kim, Chang-Jin
细胞在其原生微环境中与三维（3D）纳米特征相互作用。尽管有许多关于基底纳米形貌对细胞影响的报道，但尚未研究3D参数的独立影响。最近的进展nanofabrimetry精确控制的纳米结构的图案，周期性，形状和高度，使这个系统的研究细胞相互作用的三维纳米形貌。创建了具有不同三维度（纳米结构高度为50-600 nm）的两种不同的纳米片（柱和格栅），同时保持图案周期性（间距为230 nm）和尖端形状（针状或刀片状尖锐尖端）。人包皮成纤维细胞在针状纳米柱上表现出显著更小的细胞尺寸和更低的增殖，并且在刀片状纳米格栅上随着对齐而增强伸长。这些现象变得更加明显的纳米地形三维（结构高度）的增加。纳米柱和纳米光栅结构为丝状伪足延伸和粘附分子复合物的形成提供了不同的接触引导，这被认为导致了观察到的独特的细胞行为。(c)2006爱思唯尔有限公司保留所有权利。
Cells in their native microenvironment interact with three-dimensional (3D) nanofeatures. Despite many reports on the effects of substrate nanotopography on cells, the independent effect of 3D parameters has not been investigated. Recent advances in nanofabrication for precise control of nanostructure pattern, periodicity, shape, and height enabled this systematic study of cell interactions with 3D nanotopographies. Two distinct nanopatterns (posts and grates) with varying three-dimensionalities (50-600 nm in nanostructure height) were created, while maintaining the pattern periodicity (230 nm in pitch) and tip shape (needle- or blade-like sharp tips). Human foreskin fibroblasts exhibited significantly smaller cell size and lower proliferation on needle-like nanoposts, and enhanced elongation with alignment on blade-like nanogrates. These phenomena became more pronounced as the nano topographical three-dimensionality (structural height) increased. The nanopost and nanograte architectures provided the distinct contact guidance for both filopodia extension and the formation of adhesion molecules complex, which was believed to lead to the unique cell behaviors observed. (c) 2006 Elsevier Ltd. All rights reserved.