Gene expression profile during the life cycle of the urochordate Ciona intestinalis
Gene expression profile during the life cycle of the urochordate Ciona intestinalis
复制标题
DOI：
10.1016/j.ydbio.2007.05.022
复制
发表时间：
2007-08-15
影响因子：
2.7
通讯作者：
Satoh, Nori
中科院分区：
文献类型：
--
作者：
Azumi, Kaoru;Sabau, Sorin V.;Satoh, Nori
最近的全基因组研究和深入的表达序列标签（EST）分析已经确定了大多数与发育相关的基因。在这项研究中，我们利用大规模寡核苷酸微阵列进一步研究和鉴定具有特定或相关表达谱的基因，我们报告了在其生命周期中表达的所有肠球菌基因中约66%的全球基因表达谱。我们根据每个基因的表达谱成功地将数据集分为5个大簇和49个亚簇。这揭示了发育和衰老阶段基因表达谱的高阶性。此外，微阵列数据与EST数据库的联合分析揭示了在特定阶段或成人特定器官中表达的基因组。该研究揭示了海鞘基因表达的复杂结构，鉴定了共表达基因群和标记基因，并对许多未表征基因的生物学作用进行了预测。这种大规模的低聚dna微阵列将有助于了解基础脊索动物在发育和衰老过程中的整体基因表达和基因网络。(C) 2007年Elsevier Inc.出版
Recent whole-genome studies and in-depth expressed sequence tag (EST) analyses have identified most of the developmentally relevant genes in the urochordate, Ciona intestinalis. In this study, we made use of a large-scale oligo-DNA microarray to further investigate and identify genes with specific or correlated expression profiles, and we report global gene expression profiles for about 66% of all the C. intestinalis genes that are expressed during its life cycle. We succeeded in categorizing the data set into 5 large clusters and 49 sub-clusters based on the expression profile of each gene. This revealed the higher order of gene expression profiles during the developmental and aging stages. Furthermore, a combined analysis of microarray data with the EST database revealed the gene groups that were expressed at a specific stage or in a specific organ of the adult. This study provides insights into the complex structure of ascidian gene expression, identifies co-expressed gene groups and marker genes and makes predictions for the biological roles of many uncharacterized genes. This large-scale oligo-DNA microarray for C. intestinalis should facilitate the understanding of global gene expression and gene networks during the development and aging of a basal chordate. (C) 2007 Published by Elsevier Inc.