Immunogenicity in chickens with orally administered recombinant chicken-borne Lactobacillus saerimneri expressing FimA and OmpC antigen of O78 avian pathogenic Escherichia coli
Immunogenicity in chickens with orally administered recombinant chicken-borne Lactobacillus saerimneri expressing FimA and OmpC antigen of O78 avian pathogenic Escherichia coli
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口服表达 O78 禽致病性大肠杆菌 FimA 和 OmpC 抗原的重组鸡源性沙林乳杆菌对鸡的免疫原性
DOI：
10.1099/jmm.0.000679
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发表时间：
2018-03-01
影响因子：
3
通讯作者：
Li, Yi-Jing
中科院分区：
文献类型：
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作者：
Ma, Sun-Ting;Ding, Guo-Jie;Li, Yi-Jing
目的。禽类大肠杆菌病给全世界的家禽生产者造成了经济损失。为了解决这个问题，我们的目标是通过乳酸菌传递系统开发一种有效的口服疫苗，用于鸡抗O78禽致病性大肠杆菌（APEC）感染。对从肉鸡肠道分离的8株乳酸菌进行体外黏附能力评价，以评估其作为递送载体的潜力。在saerimneri乳杆菌M-11表面显示了APEC与树突状细胞靶向肽（DCpep）和微折叠细胞靶向肽（Co1）融合和未融合的菌毛亚基A （FimA）和外膜蛋白C (OmpC)，并产生了pPG-ompC-fimA/M-11和pPG-ompC-fimA-Co1-DCpep/M-11疫苗组。研究了重组菌株在鸡体内的定殖情况，并评价了重组菌株在鸡体内的免疫原性和免疫保护作用。重组菌株在体内的定植量与野生型菌株无显著差异。与pPG/M-11组相比，口服疫苗组鸡血清中OmpC/ fima特异性IgG和盲肠灌洗液、鼻灌洗液和粪便中黏膜IgA水平均显著升高。经APEC CVCC1553攻毒后，pPG-ompC-fimA/M-11和pPG-ompC-fimA- co1 - dcpep /M-11口服免疫鸡的保护效果较好，但两组间无显著差异。鸡源性重组沙氏乳杆菌M-11在鸡体内表现出良好的免疫原性，提示其可能是一种很有前途的抗APEC感染的候选疫苗。而哺乳动物DCpep和Co1在鸡体内的活性不显著。
Purpose. Avian colibacillosis is responsible for economic losses to poultry producers worldwide. To combat this, we aimed to develop an effective oral vaccine for chicken against O78 avian pathogenic Escherichia coli (APEC) infection through a Lactobacillus delivery system.Methodology. Eight Lactobacillus strains isolated from the intestines of broiler chickens were evaluated based on their in vitro adherence ability to assess their potential as a delivery vector. Fimbrial subunit A (FimA) and outer-membrane protein C (OmpC) of APEC with and without fusion to dendritic cell-targeting peptide (DCpep) and microfold cell-targeting peptide (Co1) were displayed on the surface of Lactobacillus saerimneri M-11 and yielded vaccine groups (pPG-ompC-fimA/M-11 and pPG-ompC-fimA-Co1-DCpep/M-11, respectively). The colonization of the recombinant strains in vivo was assessed and the immunogenicity and protective efficacy of orally administered recombinant strains in chickens were evaluated.Results. The colonization of the recombinant strains in vivo revealed no significant differences between the recombinant and wild-type strains. Chickens orally administered with vaccine groups showed significantly higher levels of OmpC/FimA-specific IgG in serum and mucosal IgA in cecum lavage, nasal lavage and stool compared to the pPG/M-11 group. After challenge with APEC CVCC1553, better protective efficacy was observed in chickens orally immunized with pPG-ompC-fimA/M-11 and pPG-ompC-fimA-Co1-DCpep/M-11, but no significant differences were observed between the two groups.Conclusions. Recombinant chicken-borne L. saerimneri M-11 showed good immunogenicity in chickens, suggesting that it may be a promising vaccine candidate against APEC infections. However, the activity of mammalian DCpep and Co1 was not significant in chickens.