Whole cell biocatalysts: essential workers from Nature to the industry.
Whole cell biocatalysts: essential workers from Nature to the industry.
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DOI：
10.1111/1751-7915.12363
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发表时间：
2017-03
影响因子：
5.7
通讯作者：
de Carvalho CC
中科院分区：
文献类型：
--
作者：
de Carvalho CC
微生物在整个进化过程中暴露于无数的底物和环境条件下，产生了无数的代谢物和酶活性。尽管人类几个世纪以来一直在利用这些特性，但我们直到最近才学会控制它们的生产，开发具有高稳定性和高生产率的新型生物催化剂，并在新的操作条件下提高它们的产量。然而，微生物细胞仍然为酶提供了最著名的环境，在非常规培养基和恶劣的反应条件下防止蛋白质结构的构象变化，同时能够有效地再生必要的辅因子并进行级联反应。此外，一种未知的微生物可能已经产生了一种化合物，可以治愈癌症、阿尔茨海默病或杀死最具抵抗力的病原体。本文综述了在筛选理想活性和提高产量方面的最新进展。
Microorganisms have been exposed to a myriad of substrates and environmental conditions throughout evolution resulting in countless metabolites and enzymatic activities. Although mankind have been using these properties for centuries, we have only recently learned to control their production, to develop new biocatalysts with high stability and productivity and to improve their yields under new operational conditions. However, microbial cells still provide the best known environment for enzymes, preventing conformational changes in the protein structure in non‐conventional medium and under harsh reaction conditions, while being able to efficiently regenerate necessary cofactors and to carry out cascades of reactions. Besides, a still unknown microbe is probably already producing a compound that will cure cancer, Alzeihmer's disease or kill the most resistant pathogen. In this review, the latest developments in screening desirable activities and improving production yields are discussed.