Plant cell biology through the window of the highly synchronized tobacco BY-2 cell line
Plant cell biology through the window of the highly synchronized tobacco BY-2 cell line
复制标题
DOI：
10.1023/a:1009832822096
复制
发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
Kumagai, Fumi
中科院分区：
文献类型：
--
作者：
Nagata, Toshiyuki;Kumagai, Fumi
同步细胞系统是研究植物细胞生物学各个方面的理想系统。然而，迄今为止，烟草BY-2细胞系是唯一可以同步化到高水平的植物细胞系。通过阿非迪霉素处理释放BY-2细胞后，获得了从S期开始的细胞同步性，而通过阿非迪霉素随后丙苯酰菌胺处理释放后，获得了从M期开始的细胞同步性。只有通过使用快速生长的烟草BY-2细胞才能达到高水平的同步性。100-一周后就折了如果增长率变低，同步水平就会降低。描述了保持烟草BY-2细胞高生长速率的技术要点。使用这种高度同步的系统，它已经有可能证明细胞周期依赖的振荡的许多基因，如细胞周期蛋白，并表征其在细胞周期中的作用。此外，该系统还促进了细胞周期特异性事件的结构和生化分析，如成膜体的发育和皮层微管的形成。还描述了这种高度同步的细胞的其他潜在用途。
Synchronous cell systems are highly desirable for investigating various aspects of plant cell biology. However, to date, the tobacco BY-2 cell line is the only plant cell line which can be synchronized to high levels. A cell synchrony starting from S phase is obtained after release of BY-2 cells from aphidicolin treatment, while that from M phase is available after release from a sequential treatment of aphidicolin followed by propyzamide. A high level of synchrony is only attained by using rapidly growing tobacco BY-2 cells that propagate ca. 100-fold in a week. Reduced levels of synchrony result if the growth rate becomes lower. Technical notes for maintaining the high growth rate of the tobacco BY-2 cell are described. Using this highly synchronous system it has been possible to demonstrate the cell cycle-dependent oscillation of many genes, such as cyclins, and characterize their role during the cell cycle. Furthermore, this system has facilitated the structural and biochemical analysis of cell cycle specific events such as the development of the phragmoplast and the formation of cortical micro-tubules. Other potential uses of this highly synchronized cells are also described.