Non-coding RNAs: the architects of eukaryotic complexity
Non-coding RNAs: the architects of eukaryotic complexity
复制标题
DOI：
10.1093/embo-reports/kve230
复制
发表时间：
2001-11-01
期刊：
影响因子：
7.7
通讯作者：
Mattick, JS
中科院分区：
文献类型：
--
作者：
Mattick, JS
在人类中，大约98%的所有转录产物是非编码RNA。RNA介导的基因调控在高等真核生物中广泛存在，并且复杂的遗传现象，如RNA干扰、共抑制、转基因沉默、印记、甲基化，可能还有位置效应花斑和转位作用，都涉及基于RNA信号传导或与之相关的交叉途径。我认为中心法则是不完整的，内含子和其他非编码RNA已经进化到在真核生物中构成基因表达的第二个层次，这使得复杂的基因活性组合能够整合和网络化。尽管蛋白质是细胞功能的基本效应物，但真核生物复杂性和表型变异的基础可能主要在于一种由高度并行的反式作用RNA系统组成的控制架构，该系统通过染色质重塑、RNA - DNA、RNA - RNA和RNA -蛋白质相互作用来传递基因表达的协调和调节所需的状态信息。这个系统与小世界网络和数据流计算有着有趣且可能具有启发性的相似之处。
Around 98% of all transcriptional output in humans is noncoding RNA. RNA-mediated gene regulation is widespread in higher eukaryotes and complex genetic phenomena like RNA interference, co-suppression, transgene silencing, imprinting, methylation, and possibly position-effect variegation and transvection, all involve intersecting pathways based on or connected to RNA signaling. I suggest that the central dogma is incomplete, and that intronic and other non-coding RNAs have evolved to comprise a second tier of gene expression in eukaryotes, which enables the integration and networking of complex suites of gene activity. Although proteins are the fundamental effectors of cellular function, the basis of eukaryotic complexity and phenotypic variation may lie primarily in a control architecture composed of a highly parallel system of trans-acting RNAs that relay state information required for the coordination and modulation of gene expression, via chromatin remodeling, RNA-DNA, RNA-RNA and RNA-protein interactions. This system has interesting and perhaps informative analogies with small world networks and dataflow computing.