Effects of dental composite resin monomers on dental pulp cells
Effects of dental composite resin monomers on dental pulp cells
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DOI：
10.4012/dmj.2018-163
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发表时间：
2019-08-01
影响因子：
2.5
通讯作者：
Lobner, Doug
中科院分区：
文献类型：
--
作者：
Schneider, Thomas R.;Hakami-Tafreshi, Roxan;Lobner, Doug
在许多牙科材料中发现的甲基丙烯酸酯单体会对牙髓细胞产生毒性，但其毒性机制尚不清楚。我们使用培养的人牙髓细胞来测试三种常用单体的效果;双酚A-甲基丙烯酸缩水甘油酯（Bis-GMA），聚氨酯二甲基丙烯酸酯（UDMA）和三甘醇二甲基丙烯酸酯（TEGDMA）。毒性大小顺序为Bis-GMA>UDMA>TEGDMA。毒性与胱氨酸摄取呈负相关，TEGDMA刺激摄取，Bis-GMA和UDMA抑制摄取。Bis-GMA和UDMA诱导氧化应激，而TEGDMA没有。毒性与谷胱甘肽水平相关性较差，因为所有化合物均降低细胞谷胱甘肽。TEGDMA的毒性低于Bis-GMA和UDMA，这可能是因为它刺激胱氨酸摄取并且不诱导氧化应激，胱氨酸摄取的增强似乎补偿了TEGDMA与谷胱甘肽的直接相互作用。Bis-GMA和UDMA都消耗谷胱甘肽并抑制胱氨酸摄取，导致氧化应激和细胞死亡。
Methacrylate monomers found in many dental materials cause toxicity to dental pulp cells but the mechanism of the toxicity is poorly understood. We used cultured human dental pulp cells to test the effects of three commonly used monomers; bisphenol-A-glycidyl methacrylate (Bis-GMA), urethane dimethacrylate (UDMA), and triethyleneglycol dimethacrylate (TEGDMA). The order of toxicity was Bis-GMA>UDMA>TEGDMA. The toxicity correlated inversely with cystine uptake, with TEGDMA stimulating uptake and Bis-GMA and UDMA inhibiting uptake. Bis-GMA and UDMA induced oxidative stress, while TEGDMA did not. Toxicity correlated poorly with glutathione levels, as all compounds decreased cellular glutathione. TEGDMA is less toxic than Bis-GMA and UDMA likely because it stimulates cystine uptake and does not induce oxidative stress, the enhanced uptake of cystine appears to compensate for TEGDMA's direct interaction with glutathione. Bis-GMA and UDMA both deplete glutathione and inhibit cystine uptake leading to oxidative stress and cell death.