Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging.
Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging.
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使用拉曼光谱成像可视化分化吸引子的外观和消失。
DOI：
10.1038/srep11358
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发表时间：
2015-06-16
影响因子：
4.6
通讯作者：
Fujita H
中科院分区：
文献类型：
--
作者：
Ichimura T;Chiu LD;Fujita K;Machiyama H;Kawata S;Watanabe TM;Fujita H
利用拉曼光谱成像，我们可视化了细胞在分化过程中的状态转变，并在由与拉曼光谱形状相关的参数组成的状态空间上构建了细胞态吸引子的假设势景。作为分化模型，我们使用了肌源性C2C12细胞系和小鼠胚胎干细胞。拉曼光谱成像可以验证描述细胞状态的多种细胞成分的数量和位置，例如蛋白质、核酸和脂类；因此，它可以报告单个细胞的状态。在这里，我们使用细胞核的拉曼光谱成像结合主成分分析来可视化细胞在分化过程中的状态转变。在分化过程中，分化前细胞状态看似均一的细胞群体在分化早期表现出异质性。在分化后期，细胞恢复到与未分化状态不同的同质细胞状态。因此，拉曼光谱成像使我们能够说明吸引子在分化环境中的消失和再现，其中细胞在分化的早期阶段在不同状态之间随机波动。
Using Raman spectral imaging, we visualized the cell state transition during differentiation and constructed hypothetical potential landscapes for attractors of cellular states on a state space composed of parameters related to the shape of the Raman spectra. As models of differentiation, we used the myogenic C2C12 cell line and mouse embryonic stem cells. Raman spectral imaging can validate the amounts and locations of multiple cellular components that describe the cell state such as proteins, nucleic acids, and lipids; thus, it can report the state of a single cell. Herein, we visualized the cell state transition during differentiation using Raman spectral imaging of cell nuclei in combination with principal component analysis. During differentiation, cell populations with a seemingly homogeneous cell state before differentiation showed heterogeneity at the early stage of differentiation. At later differentiation stages, the cells returned to a homogeneous cell state that was different from the undifferentiated state. Thus, Raman spectral imaging enables us to illustrate the disappearance and reappearance of an attractor in a differentiation landscape, where cells stochastically fluctuate between states at the early stage of differentiation.
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影响因子：
--
作者：
Bhattacharya S;Zhang Q;Andersen ME
通讯作者：
Andersen ME
影响因子：
14.9
作者：
Calvanese V;Fernández AF;Urdinguio RG;Suárez-Alvarez B;Mangas C;Pérez-García V;Bueno C;Montes R;Ramos-Mejía V;Martínez-Camblor P;Ferrero C;Assenov Y;Bock C;Menendez P;Carrera AC;Lopez-Larrea C;Fraga MF
通讯作者：
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影响因子：
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作者：
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通讯作者：
Smith, Austin G.
遗传电路的潜在景观决定了干细胞分化的时间之箭
影响因子：
3.4
作者：
Wang, Jin;Xu, Li;Huang, Sui
通讯作者：
Huang, Sui
影响因子：
48
作者：
Cloonan, Nicole;Forrest, Alistair R. R.;Grimmond, Sean M.
通讯作者：
Grimmond, Sean M.