CDNA CLONING AND MOLECULAR ANALYSIS OF 2 SELF-INCOMPATIBILITY ALLELES FROM APPLE
CDNA CLONING AND MOLECULAR ANALYSIS OF 2 SELF-INCOMPATIBILITY ALLELES FROM APPLE
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DOI：
10.1007/bf00019317
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发表时间：
1995-02-01
影响因子：
5.1
通讯作者：
BROEKAERT, WF
中科院分区：
文献类型：
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作者：
BROOTHAERTS, W;JANSSENS, GA;BROEKAERT, WF
以代表苹果自交不亲和性（S）基因座的两个等位基因的互补DNA克隆为材料，研究了苹果自交不亲和性的遗传机制。已经被分离出来并鉴定了其中一个等位基因对应于从雌蕊组织中纯化的29 kDa核糖核酸酶（S-RNase）。在北方印迹中，两种cDNA均与仅存在于雌蕊中而不存在于所分析的其它植物组织中的转录物杂交。经PCR扩增，其基因组序列分别含有138 bp和1100 bp的内含子。两个序列的比较表明，cDNA编码的成熟蛋白含有65%的相同残基。八个不变的半胱氨酸残基，两个组氨酸周围的保守区域被认为在RNA催化中发挥作用，以及许多其他不同的残基在苹果S-RNases和茄科中的类似蛋白质之间是保守的。由于这是首次报道来自与茄科无关的科的物种的S-等位基因序列，因此讨论了从其序列比较中推导出的S-RNase的结构特征。
Complementary DNA clones representing two alleles of the self-incompatibility (S) locus of apple (Malus x domestica Borkh.) have been isolated and characterised. One of the alleles corresponds to a 29 kDa ribonuclease (S-RNase) that was purified from pistil tissue. On northern blots, both cDNAs hybridized to a transcript that was only present in pistils and not in the other plant tissues analysed. Corresponding genomic sequences, amplified by PCR, were found to contain a single intron of 138 bp and 1100 bp respectively. Comparison of both sequences shows that the cDNAs encode mature proteins containing 65% of identical residues. Eight invariable cysteine residues, conserved regions around two histidines thought to play a role in RNA catalysis, and a number of other distinct residues are conserved between the apple S-RNases and similar proteins in the family Solanaceae. As this is the first report of sequences of S-alleles from a species belonging to a family that is not related with the Solanaceae, the structural features of S-RNases deduced from a comparison of their sequences are discussed.