Azalomycin F Complex Is an Antifungal Substance Produced by Streptomyces malaysiensis MJM1968 Isolated from Agricultural Soil
Azalomycin F Complex Is an Antifungal Substance Produced by Streptomyces malaysiensis MJM1968 Isolated from Agricultural Soil
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DOI：
10.3839/jksabc.2010.084
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发表时间：
2010-10-01
影响因子：
--
通讯作者：
Suh, Joo-Won
中科院分区：
文献类型：
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作者：
Cheng, Jinhua;Yang, Seung Hwan;Suh, Joo-Won
MJM 1968是一株具有较强抗真菌活性的放线菌，经16 S rDNA序列分析鉴定为马来西亚链霉菌（Streptomyces malaysiensis）。MJM 1968对枯萎病菌、立枯丝核菌、枝孢霉、厚垣孢镰刀菌、胶孢炭疽菌、苹果链格孢菌和盘多毛孢等植物病原真菌具有较强的抑菌活性。KACC 40501，体外。从菌株MJM 1968的培养滤液中分离得到一种抗真菌化合物。用菌株MJM 1968菌丝体处理农业土壤在14天后使天然真菌种群减少超过60%。用10 mg/mL部分纯化的提取物处理土壤14天后，天然真菌密度降低了80%以上。该化合物在较宽的pH和温度范围内表现出抗真菌活性，通过H-1和C-13 NMR鉴定为阿扎霉素F复合物。这是首次从S. Malaysiensis，证明了对农业土壤中真菌病原体的广谱抑制。
MJM1968, an actinomycete showing potent antifungal activity, was isolated and identified by 16S rDNA sequence analysis as Streptomyces malaysiensis. MJM1968 showed strong antifungal activity on phytopathogenic fungi such as Fusarium oxysporum, Rhizoctonia solani, Cladosporium cladosporioides, Fusarium chlamydosporum, Colletotrichum gloeosporioides KACC 40693, Alternaria mali KACC 40026, and Pestalotia spp. KACC 40501, in vitro. An antifungal compound was isolated from culture filtrate of strain MJM1968 by a series of chromatographic methods. Treatment of agricultural soil with strain MJM1968 mycelia reduced the native fungal population by more than 60% after 14 days. Treatment of soil with 10 mg/mL partially purified extract lowered native fungal density by more than 80% after 14 days. The compound, which exhibited antifungal activity in a broad range of pH and temperature, was identified as azalomycin F complex by H-1 and C-13 NMRs. This is the first report on the isolation of azalomycin F complex from S. malaysiensis, demonstrating a broad-spectrum suppression of fungal pathogens in agricultural soil.