Relationships of a transforming growth factor-β2 single nucleotide polymorphism and messenger ribonucleic acid abundance with bone and production traits in chickens
Relationships of a transforming growth factor-β2 single nucleotide polymorphism and messenger ribonucleic acid abundance with bone and production traits in chickens
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DOI：
10.1093/ps/86.5.829
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发表时间：
2007-05-01
期刊：
影响因子：
4.4
通讯作者：
Spurlock, D. M.
中科院分区：
文献类型：
--
作者：
Bennett, A. K.;Hester, P. Y.;Spurlock, D. M.
骨质疏松症是蛋鸡养殖业面临的一个严重问题，对经济、生产和福利都有影响。转化生长因子-β 2（TGF β 2）已被认为是骨重建中骨吸收和骨形成耦合的重要因素。本研究旨在确定TGF β 2是否与鸡骨矿化的变化相关，使用2种互补的实验方法。首先，在F-2群体中研究了TGF β 2中存在的内含子单核苷酸多态性（SNP），以确定其与对蛋鸡和肉鸡产业重要的骨、生长和蛋性状的关联。TGF β 2基因多态性与骨密度和骨含量显著相关（P < 0.05）.然而，当BW作为协变量纳入分析时，这些相关性变得不显著。TGF β 2 SNP与1 ~ 6周龄体重和46 ~ 55周龄产蛋量显著相关（P < 0.05）。为进一步探讨TGF β 2与骨强度的关系，比较了15、35和60周龄肉鸡和蛋鸡骨髓TGF β 2 mRNA丰度。骨和蛋性状进行了测量沿着与mRNA丰度在每个年龄段，并发现显着不同的线。在15周龄时，肉鸡的TGF β 2 mRNA丰度约为蛋鸡的4倍，但在以后的年龄，品系之间相似。因此，即使TGF β 2 SNP可能不是独立于BW变化的改善骨强度的有效标志物，但需要进一步研究TGF β 2 mRNA丰度与产蛋鸡骨强度的关系。
Osteoporosis is a serious problem for the laying hen industry with economic, production, and welfare consequences. Transforming growth factor-beta 2 (TGF beta 2) has been implicated as an important factor in coupling bone resorption and formation in bone remodeling. The current study was designed to determine if TGF beta 2 was associated with variation in bone mineralization in chickens, using 2 complementary experimental approaches. First, an intronic single nucleotide polymorphism (SNP) present in TGF beta 2 was investigated in an F-2 population to determine its association with bone, growth, and egg traits of importance to the layer and broiler industries. The TGF beta 2 SNP was significantly associated (P < 0.05) with bone mineral density and content. However, these associations became nonsignificant when BW was included as a covariate in analyses. The TGF beta 2 SNP was also significantly associated (P < 0.05) with BW from I to 6 wk of age and egg production from 46 to 55 wk of age. To further explore the relationship between TGF beta 2 and bone strength, bone marrow TGF beta 2 mRNA abundance was compared between broiler and layer chickens at 15, 35, and 60 wk of age. Bone and egg traits were measured along with mRNA abundance at each age and found to differ significantly between lines. The TGF beta 2 mRNA abundance was approximately 4-fold greater in broiler compared with layer hens at 15 wk of age but was similar between lines at later ages. Thus, even though the TGF beta 2 SNP will likely not be an effective marker for improving bone strength independently of changes in BW, further research is warranted to investigate the relationship of TGF beta 2 mRNA abundance to bone strength in laying hens.