The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators.
The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators.
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ABBA基序结合APC/C激活剂，并由APC/C底物和调节器共享。
DOI：
10.1016/j.devcel.2015.01.003
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发表时间：
2015-02-09
影响因子：
11.8
通讯作者：
Pines J
中科院分区：
文献类型：
--
作者：
Di Fiore B;Davey NE;Hagting A;Izawa D;Mansfeld J;Gibson TJ;Pines J
APC/C是一种泛素连接酶，在纺锤体组装检查点(SAC)的控制下，通过靶向特定的蛋白质在特定的时间降解来调节有丝分裂。APC/C如何识别不同的底物是控制细胞分裂的关键问题。在这里，我们已经在Cyclin A、BUBR1、Bub1和Acm1中鉴定了ABBA基序，并表明它与APC/C共激活因子CDC20结合。Cyclin A中的ABBA基序与BUBR1竞争CDC20上的同一位点，是其在前中期适当降解所必需的。此外，BUBR1和Bub1中的ABBA基序对于SAC充分发挥作用和招募CDC20到动点是必要的。因此，我们已经确定了一个保守的基序，它与有丝分裂的适当控制有关，它将APC/C底物识别与SAC联系起来。
The APC/C is the ubiquitin ligase that regulates mitosis by targeting specific proteins for degradation at specific times under the control of the Spindle Assembly Checkpoint (SAC). How the APC/C recognises its different substrates is a key problem in the control of cell division. Here, we have identified the ABBA motif in Cyclin A, BUBR1, BUB1 and Acm1, and show that it binds to the APC/C co-activator CDC20. The ABBA motif in Cyclin A is required for its proper degradation in prometaphase through competing with BUBR1 for the same site on CDC20. Moreover, the ABBA motifs in BUBR1 and BUB1 are necessary for the SAC to work at full strength and to recruit CDC20 to kinetochores. Thus, we have identified a conserved motif integral to the proper control of mitosis that connects APC/C substrate recognition with the SAC.