Occurrence of Dermatomycoses and In-Vitro Therapeutic Efficacy of Three Antifungal Drugs on the Growth of Epidermophyton Floccosum
Occurrence of Dermatomycoses and In-Vitro Therapeutic Efficacy of Three Antifungal Drugs on the Growth of Epidermophyton Floccosum
复制标题
皮肤真菌病的发生及三种抗真菌药物对絮状表皮癣菌生长的体外治疗效果
作者：
V. Awoderu;G. Nebo;V. O. Otudero
研究了皮肤真菌病的发生及几种抗真菌药物对皮肤真菌病的体外治疗效果。在接受筛查的550名小学生中，发病率为100人（18%），其中70人为男性（占接受筛查的男性的20%），30人为女性（占抽样女性的15%）。男女患病率差异不显著。分离鉴定了3种皮癣菌。分别为犬小孢子菌、疣毛菌和絮状表皮菌。抑菌剂主要有灰黄霉素、特比萘芬和酮康唑。它们对絮凝乳杆菌的生长产生了不同大小的抑制区。灰黄霉素浓度为63.00 mg/L时，对绒球菌的生长抑制率为50%。另一方面，特比萘芬在不同浓度下表现出不同程度的生长抑制，0.07 mg/L时，特比萘芬对大肠杆菌的生长抑制率达到46%。在61.81 mg/L的浓度下，该药物对分离物的生长抑制率达到100%。以酮康唑为例，100 mg/L时对生长的抑制率为50%，200 mg/L时对生长的抑制率为100%。通过肉汤稀释试验证实了三种药物的抑菌作用，在0.013 ~ 1.700 mg/L浓度范围内，特比萘芬对絮凝杆菌的生长有抑制作用，在0.027 ~ 1.700 mg/L浓度范围内，特比萘芬对絮凝杆菌有杀真菌作用。酮康唑浓度为0.003 ~ 1.700 mg/L时对絮凝乳菌的生长有抑制作用，杀真菌剂浓度为0.027 ~ 1.700 mg/L。然而，在相同的浓度范围内，它不能成功地杀死分离物。灰黄霉素在0.013 ~ 1.700 mg/L浓度下对大肠杆菌的生长有抑制作用。综上所述，酮康唑和灰黄霉素对病原菌有抑菌作用，而特比萘芬对病原菌既有抑菌作用，也有杀菌作用。特比萘芬是抑菌效果最好的药物。
The occurrence of dermatomycoses and the in-vitro therapeutic efficacy of some antifungal agents on dermatomycotic organisms were investigated. Of the 550 primary school children screened, the incidence was one hundred (18%),70 were males (representing 20% of the males screened) and 30 females (15% of the females sampled). The differences between male and female prevalence were insignificant. Three species of dermatophytes were isolated and identified. These were Microsporum canis, Trichophyton tonsurans and Epidermophyton floccosum.The antifungal agents tested on E. floccosum were griseofulvin, terbinafine and ketoconazole. They produced different sized zones of inhibition against the growthof E. floccosum. Griseofulvin exhibited a 50% inhibition of growth on E. floccosum at 63.00 mg/L.Terbinafine on the other hand exhibited varying levels of inhibition of growth at varying concentrations, at 0.07 mg/L, terbinafine achieved 46% inhibition of growth on E. floccosum. The drug achieved 100% inhibition of growth on the isolate at 61.81 mg/L.In the case of ketoconazole, 50% inhibition of growth was achieved at 100 mg/L while 100% inhibition of growth was achieved at 200 mg/L.The antifungal effects of the three drugs were confirmed by broth dilution tests where terbinafine was found to be fungistatic on the growth of E. floccosum at concentrations ranging from 0.013–1.700 mg/L and was fungicidal atconcentrations ranging from 0.027–1.700 mg/L. Ketoconazole was found to inhibit the growth of E. floccosum at 0.003– 1.700 mg/L and was fungicidalat concentrations ranging from 0.027–1.700 mg/L. It however did not succeed in killing the isolate under the same range of concentrations. Griseofulvin exhibited fungistatic effects on the growth of E. floccusum at 0.013–1.700 mg/L.In conclusion, ketoconazole and griseofulvin were found to be fungistatic and not fungicidal while terbinafine was both fungistatic and fungicidal on the pathogen. Terbinafine was found to be the most effective drug in inhibiting E. floccosum.