Soy Formula and Epigenetic Modifications: Analysis of Vaginal Epithelial Cells from Infant Girls in the IFED Study.
Soy Formula and Epigenetic Modifications: Analysis of Vaginal Epithelial Cells from Infant Girls in the IFED Study.
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DOI：
10.1289/ehp428
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发表时间：
2017-03
影响因子：
10.4
通讯作者：
Taylor JA
中科院分区：
文献类型：
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作者：
Harlid S;Adgent M;Jefferson WN;Panduri V;Umbach DM;Xu Z;Stallings VA;Williams CJ;Rogan WJ;Taylor JA
早期接触雌激素化合物会影响啮齿动物模型和人类生殖系统的发育。含有染料木素等植物雌激素的豆制品是食用大豆配方奶粉的婴儿接触源之一，它们会导致血清浓度升高。我们的目的是确定大豆暴露是否与大豆喂养的女婴阴道细胞的差异DNA甲基化有关。使用Illumina HumanMethylation450芯片，我们评估了婴儿喂养和早期发育（IFED）研究中4名大豆配方喂养和6名奶牛配方喂养女孩阴道细胞的表观基因组DNA甲基化。利用焦磷酸测序技术，我们对从出生到9个月大的50名女孩（28名大豆配方奶喂养，22名奶牛配方奶喂养）连续收集的214个阴道细胞样本中两个差异最大的甲基化位点进行了随访。通过小鼠模型，我们检测了新生儿暴露于染料木素对阴道组织中基因特异性mRNA水平的影响。全表观基因组扫描显示，大豆配方奶粉喂养的婴儿和奶牛配方奶粉喂养的婴儿在富含脯氨酸的5样基因（PRR5L）的三个CpGs甲基化上存在差异（p < 104）。对两个喂养组的焦磷酸测序发现，甲基化水平随着年龄的增长而逐渐分化，126天后，点式差异变得具有统计学意义。与对照组相比，暴露于染料木素的小鼠阴道Prr5l mRNA水平下降了50%。食用大豆配方奶粉的女孩阴道细胞DNA的DNA甲基化发生了改变，这可能与雌激素反应基因的表达减少有关。Harlid S, agent M, Jefferson WN, Panduri V, Umbach DM, Xu Z, Stallings VA, Williams CJ, Rogan WJ, Taylor JA。2017. 大豆配方和表观遗传修饰：IFED研究中女婴阴道上皮细胞的分析。环境卫生观察125:447-452；http://dx.doi.org/10.1289/EHP428
Early-life exposure to estrogenic compounds affects the development of the reproductive system in rodent models and humans. Soy products, which contain phytoestrogens such as genistein, are one source of exposure in infants fed soy formula, and they result in high serum concentrations. Our goal was to determine whether soy exposure is associated with differential DNA methylation in vaginal cells from soy-fed infant girls. Using the Illumina HumanMethylation450 BeadChip, we evaluated epigenome-wide DNA methylation in vaginal cells from four soy formula–fed and six cow formula–fed girls from the Infant Feeding and Early Development (IFED) study. Using pyrosequencing we followed up the two most differentially methylated sites in 214 vaginal cell samples serially collected between birth and 9 months of age from 50 girls (28 soy formula–fed and 22 cow formula–fed). With a mouse model, we examined the effect of neonatal exposure to genistein on gene specific mRNA levels in vaginal tissue. The epigenome-wide scan suggested differences in methylation between soy formula–fed and cow formula–fed infants at three CpGs in the gene proline rich 5 like (PRR5L) (p < 104). Pyrosequencing of the two feeding groups found that methylation levels progressively diverged with age, with pointwise differences becoming statistically significant after 126 days. Genistein-exposed mice showed a 50% decrease in vaginal Prr5l mRNA levels compared to controls. Girls fed soy formula have altered DNA methylation in vaginal cell DNA which may be associated with decreased expression of an estrogen-responsive gene. Harlid S, Adgent M, Jefferson WN, Panduri V, Umbach DM, Xu Z, Stallings VA, Williams CJ, Rogan WJ, Taylor JA. 2017. Soy formula and epigenetic modifications: analysis of vaginal epithelial cells from infant girls in the IFED study. Environ Health Perspect 125:447–452; http://dx.doi.org/10.1289/EHP428