Effects of multigrooved surf aces on osteoblast-like cells in vitro:: Scanning electron microscopic observation and mRNA expression of osteopontin and osteocalcin
Effects of multigrooved surf aces on osteoblast-like cells in vitro:: Scanning electron microscopic observation and mRNA expression of osteopontin and osteocalcin
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DOI：
10.1002/jbm.a.10158
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发表时间：
2004-02-01
影响因子：
4.9
通讯作者：
Inoue, T
中科院分区：
文献类型：
--
作者：
Matsuzaka, K;Yoshinari, M;Inoue, T
本研究评估了成骨细胞样细胞在由微槽和大槽组合组成的多槽表面上的行为。聚苯乙烯基板制造与多槽与90度，V形微槽与梯形宏槽，其具有50 μ m的脊宽度，50 μ m的壁宽度，50 μ m的底部宽度，和25 μ m的深度上的2 μ m的间距切割。还制备光滑的聚苯乙烯基底作为对照。将大鼠骨髓细胞培养成骨样细胞的基板上的形态学评价，使用扫描电子显微镜，并使用定量逆转录酶-聚合酶链反应技术的骨桥蛋白和骨钙素mRNA的表达的生化评价。孵育8天后，成骨细胞样细胞平行于多槽基底上的表面槽排列。孵育16天后，沿多沟沿着产生致密的矿化细胞外基质（ECM）。多槽表面上的ECM比光滑表面上的ECM更倾向于凹槽的方向，并且ECM的椭圆形宏槽被倒置。虽然没有显着差异，骨桥蛋白和骨钙素的mRNA表达的成骨细胞样细胞上的多槽表面往往高于光滑表面。这些结果表明，多槽可用于控制矿化ECM以及细胞的方向，并且还可增强矿化ECM的产生。(C)2003 Wiley Periodicals，Inc.
This study evaluated the behavior of osteoblast-like cells on multigrooved surfaces consisting of a combination of microgrooves and macrogrooves. A polystyrene substrate was fabricated with multigrooves with 90-degree, V-shaped microgrooves with a 2-mum pitch cut on trapezoidal macrogrooves, which had a 50-mum ridge width, a 50-mum wall width, a 50-mum bottom width, and 25-mum depth. Smooth polystyrene substrates were also prepared as controls. Rat bone marrow cells were cultured as osteoblast-like cells on the substrates for morphological evaluation using a scanning electron microscope, and for biochemical evaluation using the quantitative reverse transcriptase-polymerase chain reaction technique for osteopontin and osteocalcin mRNA expression. After 8 days of incubation, the osteoblast-like cells were aligned parallel to the surface grooves on the multigrooved substrates. After 16 days of incubation, a dense mineralized extracellular matrix (ECM) was produced along the multigrooves. The ECM on the multigrooved surface appeared oriented more in the direction of the grooves than on the smooth surface, and trapezoid-shaped macrogrooves of the ECM were cast upside down. Although there were not significant differences, the osteopontin and osteocalcin mRNA expressions of the osteoblast-like cells on the multigrooved surfaces tended to be higher than on smooth surfaces. These results suggest that multigrooves could be used to control the orientation of mineralized ECM as well as of cells, and also to enhance the production of mineralized ECM. (C) 2003 Wiley Periodicals, Inc.