Oral Immunization Against Candidiasis Using Lactobacillus casei Displaying Enolase 1 from Candida albicans.
Oral Immunization Against Candidiasis Using Lactobacillus casei Displaying Enolase 1 from Candida albicans.
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DOI：
10.3797/scipharm.1404-07
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发表时间：
2014-07
影响因子：
2.5
通讯作者：
Ueda M
中科院分区：
文献类型：
--
作者：
Shibasaki S;Karasaki M;Tafuku S;Aoki W;Sewaki T;Ueda M
念珠菌病是一种常见的真菌感染，在免疫功能低下的个体中流行。本研究利用分子展示技术研制了一种抗白色念珠菌的口服疫苗。C.通过使用来自枯草芽孢杆菌的聚-γ-谷氨酸合成酶复合物A作为锚定蛋白，在干酪乳杆菌细胞表面上表达白色念珠菌。展示Eno 1 p的L.用干酪细胞免疫小鼠，随后用致死剂量的C.白色念珠菌数据表明，疫苗引起强烈的IgG应答，并增加了接种疫苗的小鼠的存活率。此外，L.酪蛋白作为一种有效的佐剂，诱导的高抗体滴度与强佐剂如霍乱毒素诱导的抗体滴度相当。总体而言，分子展示方法可用于快速开发疫苗，其可方便地施用并且需要最少的处理。
Candidiasis is a common fungal infection that is prevalent in immunocompromised individuals. In this study, an oral vaccine against Candida albicans was developed by using the molecular display approach. Enolase 1 protein (Eno1p) of C. albicans was expressed on the Lactobacillus casei cell surface by using poly-gamma-glutamic acid synthetase complex A from Bacillus subtilis as an anchoring protein. The Eno1p-displaying L. casei cells were used to immunize mice, which were later challenged with a lethal dose of C. albicans. The data indicated that the vaccine elicited a strong IgG response and increased the survival rate of the vaccinated mice. Furthermore, L. casei acted as a potent adjuvant and induced high antibody titers that were comparable to those induced by strong adjuvants such as the cholera toxin. Overall, the molecular display method can be used to rapidly develop vaccines that can be conveniently administered and require minimal processing.