Pericyte transplantation improves skeletal muscle recovery following hindlimb immobilization.
Pericyte transplantation improves skeletal muscle recovery following hindlimb immobilization.
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周细胞移植可改善后肢固定后骨骼肌的恢复。
DOI：
10.1096/fj.201802580r
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Boppart,MarniD
中科院分区：
文献类型：
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作者：
Munroe,Michael;Dvoretskiy,Svyatoslav;Lopez,Amber;Leong,Jiayu;Dyle,MichaelC;Kong,Hyunjoon;Adams,ChristopherM;Boppart,MarniD
长时间卧床休息和肢体固定可引起骨骼肌质量和功能的迅速和显著丧失。肢体康复是停用一段时间后的标准做法，但活动能力可能严重受损，在特殊人群中，康复通常延迟或不完全。因此，迫切需要一种恢复肌肉质量的新方法。周细胞[神经胶质抗原2 (NG2)+CD31 - CD45 - (Lineage - [Lin -])和CD146+Lin -]显示出促进肌肉修复的能力，但在废弃后促进肌纤维生长的能力尚不清楚。在本研究中，3 - 4月龄小鼠单侧固定14 d （IM）或固定14 d后再活动14 d （RE）。通过流式细胞术和靶向基因表达分析来评估IM和RE后周细胞的数量和功能。此外，还进行了一项移植研究来评估周细胞对恢复的影响。定向分析结果显示，停用对周细胞基因表达的影响很小，但IM后NG2+Lin -周细胞数量减少（P < 0.05）。值得注意的是，周细胞移植恢复了RE后肌纤维横截面积和毛细血管/纤维比的损失，而单独使用载具时仍然存在缺陷（P = 0.01）。这些发现提供了第一个证据，表明周细胞可以有效地恢复骨骼肌萎缩后的骨骼肌质量。-Munroe， M, Dvoretskiy， S., Lopez， A., Leong， J., Dyle， M. C., Kong， H., Adams， C. M., Boppart， M. D.周细胞移植改善后肢固定后骨骼肌恢复。
Conditions of extended bed rest and limb immobilization can initiate rapid and significant loss of skeletal muscle mass and function. Physical rehabilitation is standard practice following a period of disuse, yet mobility may be severely compromised, and recovery is commonly delayed or incomplete in special populations. Thus, a novel approach toward recovery of muscle mass is highly desired. Pericytes [neuron-glial antigen 2 (NG2)+CD31−CD45− (Lineage− [Lin−]) and CD146+Lin−] demonstrate capacity to facilitate muscle repair, yet the ability to enhance myofiber growth following disuse is unknown. In the current study, 3–4-mo-old mice were unilaterally immobilized for 14 d (IM) or immobilized for 14 d followed by 14 d of remobilization (RE). Flow cytometry and targeted gene expression analyses were completed to assess pericyte quantity and function following IM and RE. In addition, a transplantation study was conducted to assess the impact of pericytes on recovery. Results from targeted analyses suggest minimal impact of disuse on pericyte gene expression, yet NG2+Lin− pericyte quantity is reduced following IM (P < 0.05). Remarkably, pericyte transplantation recovered losses in myofiber cross-sectional area and the capillary-to-fiber ratio following RE, whereas deficits remained with vehicle alone (P = 0.01). These findings provide the first evidence that pericytes effectively rehabilitate skeletal muscle mass following disuse atrophy.—Munroe, M., Dvoretskiy, S., Lopez, A., Leong, J., Dyle, M. C., Kong, H., Adams, C. M., Boppart, M. D. Pericyte transplantation improves skeletal muscle recovery following hindlimb immobilization.