DIFFERENCES IN THE EXPRESSION OF CONNEXIN GENES IN RAT HEPATOMAS IN-VIVO AND IN-VITRO
DIFFERENCES IN THE EXPRESSION OF CONNEXIN GENES IN RAT HEPATOMAS IN-VIVO AND IN-VITRO
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DOI：
10.1002/mc.2940110305
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发表时间：
1994-11-01
影响因子：
4.6
通讯作者：
PITOT, HC
中科院分区：
文献类型：
--
作者：
NEVEU, MJ;SATTLER, CA;PITOT, HC
正常大鼠肝细胞的间隙连接细胞间通讯（GJIC）涉及至少三种不同的连接蛋白（Cxs）-Cx 32、Cx 26、Cx43-取决于细胞类型、在小叶中的位置或两者。大鼠肝细胞原代培养物表达Cx 32和Cx 26，如在体内观察到的，而来自正常大鼠肝脏的细胞系（WB-F344、克隆9、RLEC和BRL）表达Cx43，并在较小程度上表达Cx 26。体外增殖的肝癌细胞缺乏GJIC和Cx表达（7777，8994，H4 IIE-C3）或通过由Cx43蛋白组成的间隙连接进行通讯（N1 S1 -67，9618 A）。分析肿瘤，导致从注射肝癌细胞到大鼠股肌中的Cx表达的差异相比，在体外生长的细胞。而肝癌细胞7777和H4 IIE-C3未能表达Cx mRNA在培养中，这些细胞移植在体内表达水平的Cx 32 mRNA的正常肝脏中的那些。然而，可检测到的Cx 32免疫染色中观察到小于5%的肿瘤细胞在体内。这些结果表明，Cx 32蛋白在7777和H4 IIE-C3肿瘤细胞中转录后下调。出乎意料的是，9618 A细胞在细胞培养中表达Cx43 mRNA和蛋白，但在体内表达Cx 32 mRNA。相反，N1 S1移植物在体内继续表达Cx43 mRNA和蛋白。与在N1 S1细胞悬浮培养物中观察到的点状Cx43染色不同，在体内N1 S1衍生的肿瘤中观察到弥漫性细胞内Cx43染色，尽管从体内生长的N1 S1肿瘤中分离的Cx43的电泳模式（43 kDa）与在悬浮细胞培养物中观察到的电泳模式（43和45 kDa）不同。因此，本文报道的研究结果表明，肝细胞瘤细胞中的Cx表达取决于细胞繁殖的环境，无论是体内还是体外。(C)1994 Wiley-Liss，Inc.
Gap-junctional intercellular communication (GJIC) in normal rat liver cells involves at least three different connexins (Cxs)-Cx32, Cx26, Cx43-depending on the cell type, position in the lobule, or both. Whereas rat hepatocyte primary cultures expressed Cx32 and Cx26 as observed in vivo, cell lines derived from normal rat liver (WB-F344, Clone 9, RLEC, and BRL) expressed Cx43 and to a lesser extent Cx26. Hepatoma cells propagated in vitro were either deficient in GJIC and Cx expression (7777, 8994, H4IIE-C3) or communicated via gap junctions composed of Cx43 protein (N1S1-67, 9618A). Analysis of neoplasms that resulted from injection of hepatoma cells into rat femoral muscle showed differences in Cx expression when compared with cells grown in vitro. Whereas hepatoma cells 7777 and H4IIE-C3 failed to express Cx mRNAs in culture, these cells transplanted in vivo expressed levels of Cx32 mRNA comparable to those in normal liver. However, detectable Cx32 immunostaining was observed in less than 5% of the neoplastic cells in vivo. These results indicate that Cx32 protein was posttranscriptionally downregulated in 7777 and H4IIE-C3 tumor cells. Unexpectedly, 9618A cells expressed Cx43 mRNA and protein in cell culture but expressed Cx32 mRNA in vivo. In contrast, N1S1 transplants continued to express Cx43 mRNA and protein in vivo. Unlike the punctate Cx43 staining observed in suspension cultures of N1S1 cells, diffuse intracellular Cx43 staining was observed in N1S1-derived neoplasms in vivo, although the electrophoretic pattern of Cx43 isolated from N1S1 tumors grown in vivo (43 kDa) was different from that observed in suspension cell cultures (43 and 45 kDa). Thus, the findings reported here demonstrate that Cx expression in hepatoma cells depends on the environment, whether in vivo or in vitro, in which the cells are propagated. (C) 1994 Wiley-Liss, Inc.