The complete mitochondrial genome of the Green Lizard Lacerta viridis viridis (Reptilia: Lacertidae) and its phylogenetic position within squamate reptiles
The complete mitochondrial genome of the Green Lizard Lacerta viridis viridis (Reptilia: Lacertidae) and its phylogenetic position within squamate reptiles
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DOI：
10.1016/j.gene.2007.02.006
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发表时间：
2007-06-01
期刊：
影响因子：
3.5
通讯作者：
Berendonk, T. U.
中科院分区：
文献类型：
--
作者：
Boehme, M. U.;Fritzsch, G.;Berendonk, T. U.
这是首次对一种蜥蜴科成员的完整线粒体基因组进行测序。为了比较该科与其他爬行动物谱系的系统发育关系，对该科的绿色文昌鱼进行了测序。利用长聚合酶链式反应(Long-PCR)，我们获得了一个17,156个碱基的线粒体基因组，显示出典型的脊椎动物模式，包括13个蛋白质编码基因，22个转移RNA(TRNA)，两个核糖体RNA(RRNA)和一个主要的非编码区。绿色乳杆菌的非编码区在其5‘端有一个明显的35bp串联重复序列。一项包括目前所有可用的鳞类线粒体序列的系统发育研究证明了蜥科在单系鳞类群中的位置。在此基础上，我们得到了短尾蛇科、鬣禽科、长尾蛇科、长尾蛇科和长尾蛇科之间的较窄的亲缘关系。虽然这个组的内部关系只产生了弱的分辨率和较低的引导支持，但所揭示的关系与形态研究比最近的分子分析更一致。(C)2007 Elsevier B.V.保留所有权利。
For the first time the complete mitochondrial genome was sequenced for a member of Lacertidae. Lacerta viridis viridis was sequenced in order to compare the phylogenetic relationships of this family to other reptilian lineages. Using the long-polymerase chain reaction (long PCR) we characterized a mitochondrial genome, 17,156 bp long showing a typical vertebrate pattern with 13 protein coding genes, 22 transfer RNAs (tRNA), two ribosomal RNAs (rRNA) and one major noncoding region. The noncoding region of L. v. viridis was characterized by a conspicuous 35 bp tandem repeat at its 5 ' terminus. A phylogenetic study including all currently available squamate mitochondrial sequences demonstrates the position of Lacertidae within a monophyletic squamate group. We obtained a narrow relationship of Lacertidae to Scincidae, Iguanidae, Varanidae, Anguidae, and Cordylidae. Although, the internal relationships within this group yielded only a weak resolution and low bootstrap support, the revealed relationships were more congruent with morphological studies than with recent molecular analyses. (C) 2007 Elsevier B.V. All rights reserved.