Simple and rapid quantitative high-performance liquid chromatographic analysis of plasma amino acids
Simple and rapid quantitative high-performance liquid chromatographic analysis of plasma amino acids
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DOI：
10.1016/j.jchromb.2007.01.002
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发表时间：
2007-06-01
影响因子：
3
通讯作者：
Powers, Robert W.
中科院分区：
文献类型：
--
作者：
Frank, Michael P.;Powers, Robert W.
建立了一种简单、快速的高效液相色谱测定血浆氨基酸的方法。该方法使用最小的样品体积，并通过邻苯二甲醛和荧光检测对氨基酸进行自动在线柱前衍生化。通过简化的梯度分离氨基酸，无需柱加热。所有氨基酸的测定在 5 至 1000 μmol/L 范围内呈线性。氨基酸回收率为 91% 至 108%，批内变异系数 (CV) 为 1-7%，批间变异系数为 2-12%。简单的样品制备和最小的样品体积使得该方法可用于患者和实验动物样品中氨基酸的定量。 (c) 2007 Elsevier B.V. 保留所有权利。
A simple and rapid high performance liquid chromatographic method for the determination of plasma amino acids was developed. The method uses minimal sample volume and automated online precolumn derivitization of amino acids with o-phthalaldehyde and fluorescent detection. Amino acids are separated by a simplified gradient without column heating. The assay is linear from 5 to 1000 mu mol/L for all amino acids. Recovery of amino acids was between 91 and 108%, intra-assay coefficient of variation (CV) was 1-7%, and inter-assay CV was 2-12%. The simple sample preparation and minimal sample volume make the method useful for the quantitation of amino acids in both patient and experimental animal samples. (c) 2007 Elsevier B.V. All rights reserved.