REARING OF HONEYBEE LARVAE INVITRO - EFFECT OF YEAST EXTRACT ON QUEEN DIFFERENTIATION
REARING OF HONEYBEE LARVAE INVITRO - EFFECT OF YEAST EXTRACT ON QUEEN DIFFERENTIATION
复制标题
DOI：
10.1080/00218839.1981.11100492
复制
发表时间：
1981-01-01
影响因子：
1.9
通讯作者：
LACKNER, B
中科院分区：
文献类型：
--
作者：
REMBOLD, H;LACKNER, B
一龄蜜蜂幼虫（60/测试）在受控条件下体外饲养。如果使用蜂王浆，用水1：1稀释并补充D-葡萄糖和D-果糖（40克试验食品中2.5克），成虫的产量为70-80％，10％为蜂王。将 Difco 细菌酵母提取物（0.5 克）或木炭处理的提取物（1 克）添加到该碱性食品中。在相同的存活率下，幼虫的生长和蜂王的确定都会增加（30% 和 50% 的蜂王）。通过凝胶色谱将木炭处理的酵母提取物分离成盐和氨基酸部分。盐成分使成虫产量降低约 50%，但增加了幼虫的生长速度和分化（20% 的蜂王）。氨基酸部分将存活率降低至.apprx。 30%；生长速度和种姓分化均低于基本食物的情况。添加两种级分得到与添加木炭处理的酵母提取物相同的结果。 4-5龄幼虫平均增重与种姓分化程度有关。对于常规测试，在受控的体外条件下，使用蜂王浆、水、糖和酵母提取物的混合物可产生 > 30% 的蜂王，一龄工蜂幼虫的存活率接近 80%。
First instar honeybee larvae (60/test) were reared in vitro under controlled conditions. The yield of adults was 70-80%, and 10% were queens if royal jelly was used, diluted 1:1 with water and supplemented with D-glucose and D-fructose (2.5 g in 40 g test food). Difco bacto-yeast extract (0.5 g) or charcoal treated extract (1 g) were added to this basic food. At the same rate of survival, larval growth and queen determination were increased (30% and 50% queens). The charcoal-treated yeast extract was separated by gel chromatography into a salt and an amino acid fraction. The salt component reduced the yield of adults to about 50% but it increased larval growth rate and differentiation (20% queens). The amino acid fraction reduced the rate of survival to .apprx. 30%; growth rate and caste differentiation were below those with the basic food. Addition of both fractions gave the same result as addition of charcoal-treated yeast extract. The average gain in weight of 4th-5th instar larvae was related to the degree of caste differentiation. For routine tests, use of a mixture of royal jelly, water, sugar and yeast extract yields > 30% queens, at a survival rate from 1st instar worker larvae near 80%, under controlled in vitro conditions.