Expression of dominant negative cadherin in the adult mouse brain modifies rearing behavior
Expression of dominant negative cadherin in the adult mouse brain modifies rearing behavior
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DOI：
10.1016/j.mcn.2003.12.005
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发表时间：
2004-03-01
影响因子：
3.5
通讯作者：
Semb, H
中科院分区：
文献类型：
--
作者：
Edsbagge, J;Zhu, SW;Semb, H
钙粘附素超家族细胞-细胞黏附分子(CAM)是神经系统发育过程中形态发生和轴突引导的重要调节因子，在大脑的神经可塑性中发挥重要作用。为了研究后者，我们建立了一个小鼠模型，在成年小鼠的大脑中表达显性负的经典钙粘附素。测试小鼠在旷野中的自发运动活动和探索行为，在正迷宫中的焦虑，在水-T迷宫中的空间学习和记忆。在野外，表达显性负性钙粘附素的小鼠的饲养行为减少，但运动活动没有变化，这表明探索行为存在缺陷。在水迷宫中，表达突变型钙粘附素的动物表现出正常的逃避潜伏期，与对照仔鼠难以区分。同样，钙粘蛋白突变小鼠和对照小鼠的海马片中的LTP也是无法区分的。这些发现表明，在表达显性负钙粘附素的小鼠中，空间学习是完整的，但改变了饲养行为，这表明经典钙粘附素参与了调节饲养行为的机制。(C)2004 Elsevier Inc.保留所有权利。
The cadherin superfamily of cell-cell adhesion molecules (CAM) are crucial regulators of morphogenesis and axonal guidance during development of the nervous system and have been suggested to play important roles in neural plasticity of the brain. To study the latter, we created a mouse model that expressed a dominant negative classical cadherin in the brain of adult mice. The mice were tested for spontaneous motor activity and exploratory behavior in the open field, anxiety in the plus-maze, and spatial learning and memory in the water-T maze. Mice expressing the dominant negative cadherin displayed reduced rearing behavior, but no change in motor activity, in the open field, indicating deficits in exploratory behavior. In the water maze, animals expressing the mutant cadherin showed normal escape latencies and were indistinguishable from control littermates. Similarly, LTP in hippocampal slices of cadherin mutant and control mice were indistinguishable. These findings demonstrate intact spatial learning in mice expressing a dominant negative cadherin but altered rearing behavior, suggesting the involvement of classical cadherins in mechanisms mediating rearing behavior. (C) 2004 Elsevier Inc. All rights reserved.