Formalin-induced changes of NMDA receptor subunit expression in the spinal cord of the rat
Formalin-induced changes of NMDA receptor subunit expression in the spinal cord of the rat
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DOI：
10.1007/s00726-001-0125-3
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发表时间：
2002-01-01
期刊：
影响因子：
3.5
通讯作者：
Allgaier, C
中科院分区：
文献类型：
--
作者：
Gaunitz, C;Schüttler, A;Allgaier, C
采用RT-PCR方法研究福尔马林对大鼠腰髓NMDA受体亚基及其剪接变异体mRNA表达的影响。在将福尔马林注射（5%;皮下注射）到Sprague-Dawley大鼠的后爪中之后，动物表现出典型的双相行为疼痛反应。福尔马林注射后6小时制备脊髓（L3-6）。在对照组中，NR 1-B的优势大于NR 1-a，NR 1 -2和NR 1 -4的优势分别大于NR 1 -1和NR 1 -3。关于对照中NR 2亚基的表达，NR 2B表现出最高的表达，其次是NR 2C、NR 2A和NR 2D的比例降低。福尔马林处理不影响NR 1剪接变异体的表达，但显着增加和减少NR 2A和NR 2C的比例，分别。总之，目前的数据表明，适应性变化的NR亚基的表达模式在大鼠脊髓由于福尔马林注射。
Using RT-PCR, the present study investigated the effects of formalin administration on mRNA expression coding for NMDA receptor (NR) subunits and splice variants in rat lumbar spinal cord. Subsequent to formalin injection (5%; subclutaneously) into the hind paw of Sprague-Dawley rats, the animals exhibited the typical biphasic behavioural pain response. Spinal cord (L3-6) was prepared six hours after formalin injection. In controls, NR1-b predominated over NR1-a, and NR1-2 and NR1-4 exceeded over NR1-1 and NR1-3, respectively. Regarding the NR2 subunit expression in controls, NR2B exhibited the highest expression, followed by decreasing proportions of NR2C, NR2A, and NR2D. Formalin treatment did not affect NR1 splice variant expression but significantly increased and decreased the proportion of NR2A and NR2C, respectively. In summary, the present data demonstrate adaptive changes in the NR subunit expression pattern in rat spinal cord due to formalin injection.