Hindbrain orexin 1 receptors influence palatable food intake, operant responding for food, and food-conditioned place preference in rats.
Hindbrain orexin 1 receptors influence palatable food intake, operant responding for food, and food-conditioned place preference in rats.
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DOI：
10.1007/s00213-013-3248-9
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发表时间：
2014-01
影响因子：
3.4
通讯作者：
Williams, Diana L.
中科院分区：
文献类型：
--
作者：
Kay, Kristen;Parise, Eric M.;Lilly, Nicole;Williams, Diana L.
关键词：
大脑食欲素1受体（OX 1 R）参与食物动机行为。大多数研究都集中在前脑OX 1 R群体，但后脑OX 1 R影响进食。我们假设，后脑OX 1 Rs影响食物的奖励价值。我们研究了刺激或阻断后脑OX 1 R对食物动机、可口高脂肪（HF）食物摄入和食物条件性位置偏好的影响。在操作性测试阶段之前，按照渐进比例（PR）计划训练杠杆按压蔗糖的大鼠接受第四次脑室内（icv）注射溶媒、食欲素-A（0.1-1 nmol）或OX 1 R拮抗剂SB 334867（10-20 nmol）。在每日1小时的测试中，这些治疗对HF食物摄入量的影响通过第四侧脑室和孤束核（NTS）注射进行评估。我们通过将HF食物与双侧腔室的一侧配对来调节位置偏好，然后研究20 nmol第四icv SB 334867对该偏好表达的影响。在PR时间表的随意喂食大鼠中，第四icv食欲素-A相对于媒介物显著增加了应答和断点。在24小时的食物剥夺大鼠，第四icv SB 334867显着降低响应和断点。Orexin-A（0.1 nmol）或NTS（0.01 nmol）输送到第四脑室增加HF饮食摄入。第四icv SB 334867不影响HF食物摄入，但SB 334867提供第四icv（20 nmol）或NTS内（5-10 nmol）抑制食物摄入。HF食物条件性位置偏爱的表达被第四icv SB 334867抑制。后脑OX 1 R活性影响食物动机的操作行为，并可能在响应预测可口食物的线索中发挥作用。
Brain orexin 1 receptors (OX1Rs) are involved in food-motivated behavior. Most research has focused on fore-brain OX1R populations, but hindbrain OX1Rs affect feeding. We hypothesized that hindbrain OX1Rs affect the reward value of food. We examined the effects of hindbrain OX1R stimulation or blockade on motivation for food, palatable high-fat (HF) food intake, and food-conditioned place preference. Rats trained to lever press for sucrose on a progressive ratio (PR) schedule received fourth intracerebroventricular (icv) injections of vehicle, orexin-A (0.1–1 nmol), or the OX1R antagonist SB334867 (10–20 nmol) before operant test sessions. Effects of these treatments on HF food intake during daily 1-h tests were assessed with fourth icv and nucleus of the solitary tract (NTS) injections. We conditioned a place preference by pairing HF food with one side of a two-sided chamber and then examined the effect of 20 nmol fourth icv SB334867 on the expression of that preference. In ad lib fed rats on the PR schedule, fourth icv orexin-A significantly increased responding and breakpoint relative to the vehicle. In 24-h food-deprived rats, fourth icv SB334867 significantly decreased responding and breakpoint. Orexin-A delivered to the fourth ventricle (0.1 nmol) or NTS (0.01 nmol) increased HF diet intake. Fourth icv SB334867 did not affect HF food intake, but SB334867 delivered either fourth icv (20 nmol) or intra-NTS (5–10 nmol) suppressed chow intake. Expression of HF food-conditioned place preference was inhibited by fourth icv SB334867. Hindbrain OX1R activity affects food-motivated operant behavior and may play a role in responding to cues that predict palatable food.
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影响因子：
3.3
作者：
Choi, D. L.;Davis, J. F.;Magrisso, I. J.;Fitzgerald, M. E.;Lipton, J. W.;Benoit, S. C.
通讯作者：
Benoit, S. C.
影响因子：
3.4
作者：
Richards, Jemma K.;Simms, Jeffrey A.;Steensland, Pia;Taha, Sharif A.;Borgland, Stephanie L.;Bonci, Antonello;Bartlett, Selena E.
通讯作者：
Bartlett, Selena E.
影响因子：
2.7
作者：
Porter, RA;Chan, WN;Austin, N
通讯作者：
Austin, N
影响因子：
4.8
作者：
Baird, John-Paul;Choe, Angela;Grigg, Lindsay A.
通讯作者：
Grigg, Lindsay A.
影响因子：
4.7
作者：
Skibicka, Karolina P.;Shirazi, Rozita H.;Dickson, Suzanne L.
通讯作者：
Dickson, Suzanne L.