Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells
Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells
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DOI：
10.1016/j.acthis.2009.05.001
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发表时间：
2010-01-01
影响因子：
2.5
通讯作者：
Matsumoto, Koukichi
中科院分区：
文献类型：
--
作者：
Masuda-Murakami, Yoshiko;Kobayashi, Miho;Matsumoto, Koukichi
以热休克蛋白25 （hsp25）作为成牙髓细胞分化的标志物，研究了三氧化二矿聚集体（MTA）对牙髓细胞成牙髓样分化的影响。用牙色MTA或含氢氧化钙水泥（Dycal）培养细胞。用激光共聚焦扫描显微镜观察材料对牙髓细胞的影响。用抗hsp25和肌动蛋白的多克隆抗体对细胞进行免疫细胞化学标记。采用定量逆转录聚合酶链反应（RT-PCR）检测2 d时牙髓细胞中hsp25和dspp mRNA的表达。MTA培养的大部分细胞对hsp25表现出强烈的免疫标记，2 d时hsp25和dspp mRNA表达量高于Dycal培养的细胞。这些结果表明MTA是一种有效的牙髓封盖材料，能够诱导成牙本质样细胞的分化和修复性三级牙本质的形成，并且凋亡最少。2009爱思唯尔有限公司版权所有。
The effect of mineral trioxide aggregate (MTA) on the odontoblast-like differentiation of pulp cells was evaluated using heat-shock protein 25 (hsp25) as a marker for odontoblast differentiation. The cells were cultured with tooth-colored MTA or calcium hydroxide-containing cement (Dycal). The effects of the materials on the pulp cells were observed using a confocal laser scanning microscope. The cells were labelled immunocytochemically using polyclonal antibodies against hsp25 and actin. The mRNA expression of hsp25 and dspp in the pulp cells at 2 days were examined by quantitative reverse transcription-polymerase chain reaction (RT-PCR). Most of the cells cultured with MTA showed an intense immunlabelling for hsp25 and the mRNA expressions of hsp25 and dspp at 2 days were higher than those cultured with Dycal. These findings indicate that MTA is an effective pulp capping material and is able to induce the differentiation of odontoblast-like cells and the formation of reparative tertiary dentin with minimum apoptosis. (C) 2009 Elsevier GmbH. All rights reserved.