The differentiation-related gene 1, Drg1, is markedly upregulated by androgens in LNCaP prostatic adenocarcinoma cells
The differentiation-related gene 1, Drg1, is markedly upregulated by androgens in LNCaP prostatic adenocarcinoma cells
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DOI：
10.1016/s0014-5793(99)00845-5
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发表时间：
1999-07-16
期刊：
影响因子：
3.5
通讯作者：
Verhoeven, G
中科院分区：
文献类型：
--
作者：
Ulrix, W;Swinnen, JV;Verhoeven, G
采用差异显示技术对LNCaP前列腺腺癌细胞中雄激素调控基因进行鉴定。被雄激素显著上调的基因之一被证明与分化相关基因1（Drg 1;也被描述为RTP、Cap 43和rit 42）相同，该基因的表达最近被证明在结肠、乳腺和前列腺肿瘤中减少。我们表明，Drg 1是大量表达（雄激素暴露）的人前列腺和它的表达刺激一些14倍的雄激素处理的LNCaP细胞。诱导的配体特异性反映了LNCaP中突变的雄激素受体的特异性改变。在雄激素受体阴性肿瘤中，Pines基础表达略高于LNCaP，但不存在诱导。这些数据表明，Drg 1是一种新的标记雄激素诱导分化的人前列腺。(C)1999年欧洲生物化学学会联合会。
A differential display technique was used to identify androgen-regulated genes in LNCaP prostatic adenocarcinoma cells. One of the genes markedly upregulated by androgens proved to be identical to differentiation-related gene 1 (Drg1; also described as RTP, Cap43 and rit42), a gene whose expression has recently been shown to be diminished in colon, breast and prostate tumors. We show that Drg1 is abundantly expressed in the (androgen-exposed) human prostate and that its expression is stimulated some 14-fold in androgen-treated LNCaP cells. The ligand specificity of the induction reflects the altered specificity of the mutated androgen receptor in LNCaP, In androgen receptor negative tumor Pines basal expression is slightly higher than in LNCaP but inducibility is absent. These data suggest that Drg1 is a novel marker of androgen-induced differentiation in the human prostate. (C) 1999 Federation of European Biochemical Societies.