INACTIVATION OF VIRUSES IN LABILE BLOOD DERIVATIVES .1. DISRUPTION OF LIPID-ENVELOPED VIRUSES BY TRI( NORMAL-BUTYL)PHOSPHATE DETERGENT COMBINATIONS
INACTIVATION OF VIRUSES IN LABILE BLOOD DERIVATIVES .1. DISRUPTION OF LIPID-ENVELOPED VIRUSES BY TRI( NORMAL-BUTYL)PHOSPHATE DETERGENT COMBINATIONS
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DOI：
10.1046/j.1537-2995.1985.25686071422.x
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
STRYKER, MH
中科院分区：
文献类型：
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作者：
HOROWITZ, B;WIEBE, ME;STRYKER, MH
通过向抗血友病因子（AHF）浓缩液中添加标记病毒（VSV，Sindbis，Sendai，EMC），评价了有机溶剂磷酸三丁酯（TNBP）和去污剂用于灭活不稳定血液衍生物中病毒的用途。用TNBP加吐温80获得的病毒灭活率上级于用乙醚加吐温80观察到的病毒灭活率，乙醚加吐温80是先前显示的使大于或等于106.9 CID 50的B型肝炎和大于或等于104 CID 50的哈钦森株非甲非B型肝炎的条件。TNBP/Tween处理后的AHF回收率大于或等于90%。反应后，TNBP可以通过Sephadex G25上的凝胶排阻色谱从蛋白质中去除;然而，由于其大的胶束尺寸。用此方法不能从蛋白质中除去吐温80。通过蛋白质的差异沉淀去除吐温80的尝试仅部分成功。另一种去污剂胆酸钠与TNBP结合使用时，几乎同样有效地灭活了病毒，AHF的回收率为80%。由于胆酸钠形成小胶束，可通过SephadexG 25层析除去。TNBP/胆酸盐处理的AHF浓缩物的电泳检查显示，除了血浆脂蛋白外，蛋白质迁移率几乎没有变化（如果有的话）。
Use of the organic solvent, tri(n-butyl)phosphate (TNBP), and detergents for the inactivation of viruses in labile blood derivatives was evaluated by addition of marker viruses (VSV, Sindbis, Sendai, EMC) to anti-hemophilic factor (AHF) concentrates. The rate of virus inactivation obtained with TNBP plus Tween 80 was superior to that observed with ethyl ether plus Tween 80, a condition previously shown to inactivate greater than or equal to 106.9 CID50 of hepatitis B and greater than or equal to 104 CID50 of Hutchinson strain non-A, non-B hepatitis. The AHF recovery after TNBP/Tween treatment was greater than or equal to 90 percent. Following the reaction, TNBP could be removed from the protein by gel exclusion chromatography on Sephadex G25; however, because of its large micelle size. Tween 80 could not be removed from protein by this method. Attempts to remove Tween 80 by differential precipitation of protein were only partially successful. An alternate detergent, sodium cholate, when combined with TNBP, resulted in almost as efficient virus inactivation and an 80 percent recovery of AHF. Because sodium cholate forms small micelles, it could be removed by Sephadex G25 chromatography. Electrophoretic examination of TNBP/cholate-treated AHF concentrates revealed few, if any, changes in protein mobility, except for plasma lipoprotein(s).