Self-assembly of zein microspheres with controllable particle size and narrow distribution using a novel built-in ultrasonic dialysis process
Self-assembly of zein microspheres with controllable particle size and narrow distribution using a novel built-in ultrasonic dialysis process
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使用新型内置超声波透析工艺自组装具有可控粒径和窄分布的玉米醇溶蛋白微球
DOI：
10.1016/j.cej.2015.09.067
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发表时间：
2016-01-15
影响因子：
15.1
通讯作者：
Jiang, Yanbin
中科院分区：
文献类型：
--
作者：
Liu, Guijin;Wei, Dongwei;Jiang, Yanbin
采用一种新型的内置超声透析工艺（BUDP）自组装了粒径可控、粒径分布窄的玉米蛋白微球。采用OA(16)(4)(5)正交实验设计研究了不同变量对所得玉米蛋白颗粒微观结构的影响。结果表明：在不同工艺参数下，BUDP法制备的玉米蛋白颗粒呈球形，平均粒径Dp（50）在431.2 +/- 69.6 nm ~ 2335 +/- 406.1 nm之间。优化条件下制备的玉米蛋白微球（Dp(50) = 398.2 +/- 41.3 nm）比无超声条件下制备的玉米蛋白微球（Dp(50) = 1776 +/- 1181.1 nm）粒径更小，粒径分布更窄。FT-IR、H-1 NMR、XRD、TG和DSC表征结果表明，BUDP法制备的玉米蛋白微球的化学结构、晶体结构和热行为与未超声法制备的玉米蛋白微球相似。超声功率和玉米蛋白浓度是影响颗粒形成最重要的工艺参数。详细讨论了玉米蛋白微球的形成机理和操作参数的影响机理。此外，还建立了预测粒径的半经验模型。此外，利用BUDP将姜黄素包埋在玉米蛋白微球中，成功地延缓了姜黄素在体外的释放速度。(C) 2015 Elsevier B.V.版权所有
Zein microspheres with controllable particle size and narrow size distribution were self-assembled using a novel built-in ultrasonic dialysis process (BUDP). The effects of different variables on the microstructures of obtained zein particles were investigated using an OA(16) (4(5)) orthogonal experimental design. The results showed that zein particles prepared by the BUDP possessed a spherical morphology with mean particle size (Dp(50)) from 431.2 +/- 69.6 nm to 2335 +/- 406.1 nm under different process parameters. Zein microspheres prepared at optimized conditions presented much smaller particle size and narrower size distribution (Dp(50) = 398.2 +/- 41.3 nm) than those obtained without ultrasound (Dp(50) = 1776 +/- 1181.1 nm). FT-IR, H-1 NMR, XRD, TG and DSC characterization results showed that the chemical structure, crystal structure and thermal behavior of the zein microspheres obtained by BUDP were similar with raw zein and that prepared without ultrasound. The ultrasound power and zein concentration were the most important process parameters affecting particle formation. The formation mechanisms of zein microspheres and the influence mechanisms of the operating parameters were discussed in detail. Also, a semi-empirical model was developed for predicting particle size. Furthermore, curcumin was encapsulated into zein microspheres using BUDP, and curcumin-loaded zein microspheres sustained curcumin release rate successfully in vitro. (C) 2015 Elsevier B.V. All rights reserved.