Perturbing nuclear transport in Drosophila eye imaginal discs causes specific cell adhesion and axon guidance defects.
Perturbing nuclear transport in Drosophila eye imaginal discs causes specific cell adhesion and axon guidance defects.
复制标题
果蝇眼成虫盘中核运输的扰动会导致特定的细胞粘附和轴突引导缺陷。
DOI：
10.1006/dbio.2001.0468
复制
发表时间：
2001
影响因子：
2.7
通讯作者：
Davis,I
中科院分区：
文献类型：
--
作者：
Kumar,JP;Wilkie,GS;Tekotte,H;Moses,K;Davis,I
为了研究多细胞发育过程中核质的转运，我们建立了一种灵敏的活胚盘胚胎核蛋白输入试验。我们发现，在果蝇中，人核转运受体karyopherinβ/Importinβ（DNImpβ）的显性负截短破坏mRNA输出和蛋白质输入。为了测试不同发育过程对核运输扰动的敏感性，我们在眼盘的形态发生沟后面表达DNImpβ，此时光感受器被图案化并将其轴突投射到大脑。DNImpβ表达不会破坏不同光感受器的正确特化，但会导致细胞粘附缺陷，导致一些光感受器下降到小眼层以下。光感受器最初将其轴突正确地投射到后部，但后来它们的轴突无法进入通往大脑的视柄，并继续投射广泛的误导轴突网络。轴突导向和细胞粘附缺陷都是由于Ketel功能的破坏，Ketel是Importinβ的果蝇直系同源物。我们的结论是，细胞粘附和轴突在眼睛的指导有特定的要求，核质运输，尽管涉及的过程主要发生在细胞表面。
To study nucleocytoplasmic transport during multicellular development, we developed a sensitive nuclear protein import assay in living blastoderm embryos. We show that dominant negative truncations of the human nuclear transport receptor karyopherinβ/Importinβ (DNImpβ) disrupt mRNA export and protein import in Drosophila. To test the sensitivity of different developmental processes to nuclear trafficking perturbations, we expressed DNImpβ behind the morphogenetic furrow of the eye disc, at a time when photoreceptors are patterned and project their axons to the brain. DNImpβ expression does not disrupt the correct specification of different photoreceptors, but causes a defect in cell adhesion that leads to some photoreceptors descending below the layer of ommatidia. The photoreceptors initially project their axons correctly to the posterior, but later their axons are unable to enter the optic stalk en route to the brain and continue to project an extensive network of misguided axons. The axon guidance and cell adhesion defects are both due to a disruption in the function of Ketel, the Drosophila ortholog of Importinβ. We conclude that cell adhesion and axon guidance in the eye have specific requirements for nucleocytoplasmic transport, despite involving processes that occur primarily at the cell surface.