Impact of dietary oils and fats on lipid peroxidation in liver and blood of albino rats
Impact of dietary oils and fats on lipid peroxidation in liver and blood of albino rats
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DOI：
10.1016/s2221-1691(14)60208-2
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发表时间：
2014-01-01
影响因子：
1.7
通讯作者：
Ramadan, Mohamed Fawzy
中科院分区：
文献类型：
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作者：
Haggag, Mohammad El-Sayed Yassin El-Sayed;Elsanhoty, Rafaat Mohamed;Ramadan, Mohamed Fawzy
目的：探讨不同膳食油脂（不同饱和度和不饱和度）对大鼠肝脏和血液脂质过氧化的影响。方法：将50只白化大鼠随机分为5组，每组10只。这些组用膳食黄油（第一组）、人造黄油（第二组）、橄榄油（第三组）、葵花籽油（第四组）和玉米油（第五组）喂养7周。停止饮食12 It后，切除肝脏并收集血液以测量肝匀浆上清液和血液中的丙二醛(MDA)水平。还测量了血液超氧化物歧化酶活性（SOD）、谷胱甘肽过氧化物酶活性（GPx）、血清维生素E和总抗氧化能力（TAC）水平，以确定油脂对脂质过氧化的影响。结果：结果表明，五组之间SOD活性、维生素E和TAC水平没有观察到显着差异。然而，与其他组相比，IV组和V组的GPx活性显着降低。结果表明，与其他油脂相比，饲喂玉米油会导致肝脏和血液中 MDA 水平显着升高。 SOD 与 GPx、维生素 E 与 TAC、GPx 与 TAG 均呈正相关（r：0.743；P
Objective: To investigate the effects of different dietary fat and oils (differing in their degree of saturation and unsaturation) on lipid peroxidation in liver and blood of rats.Methods: The study was conducted on 50 albino rats that were randomly divided into 5 gaups of 10 animals. The groups were fed on dietary butter (Group I), margarine (Group II), olive oil (Group III), sunflower oil (Group IV) and corn oil (Group V) for 7 weeks. After 12 It of diet removal, livers were excised and blood was collected to measure malondialdehyde (MDA) levels in the supernatant of liver homogenate and in blood. Blood superoxide dismutase activity (SOD), glutathione peroxidase activity (GPx), serum vitamin E and total antioxidant capacity (TAC) levels were also measured to determine the effects of fats and oils on lipid peroxidation.Results: The results indicated that no significant differences were observed in SOD activity, vitamin E and TAC levels between the five groups. However, there was significant decrease of GPx activity in groups IV and V when compared with other groups. The results indicated that feeding corn oil caused significant increases in liver and blood MDA levels as compared with other oils and fats. There were positive correlations between SOD and GPx, vitamin E and TAC as well as between GPx and TAG (r: 0.743; P