Changes in pericytic expression of NG2 and PDGFRB and vascular permeability in the sensory circumventricular organs of adult mouse by osmotic stimulation
Changes in pericytic expression of NG2 and PDGFRB and vascular permeability in the sensory circumventricular organs of adult mouse by osmotic stimulation
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DOI：
10.1002/cbf.2971
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发表时间：
2014-01
影响因子：
3.6
通讯作者：
S. Morita;Atsushi Hourai;S. Miyata
中科院分区：
文献类型：
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作者：
S. Morita;Atsushi Hourai;S. Miyata
血脑屏障（BBB）是一种屏障，可防止血液来源的物质通过紧密连接自由进入大脑并维持专门的大脑环境。室周器官 (CVO) 缺乏典型的 BBB。感觉 CVO 的有窗脉管系统，包括终板血管器 (OVLT)、穹窿下器官 (SFO) 和后区 (AP)，使实质细胞能够感知各种血液来源的信息，包括渗透信息。在本研究中，我们利用免疫组织化学方法检测慢性渗透刺激期间成年小鼠 OVLT、SFO 和 AP 中 NG2 和血小板衍生生长因子受体 β (PDGFRB) 表达的变化。 NG2 和 PDGFRB 在周细胞中的表达非常显着，尽管这些血管生成相关蛋白在发育中的未成熟脉管系统的周细胞中高度表达。慢性盐负荷显着增加OVLT和SFO中NG2的表达以及OVLT、SFO和AP中PDGFRB的表达。 OVLT 和 SFO 中的慢性盐负荷显着增加了低分子质量示踪剂异硫氰酸荧光素的血管通透性，但 AP 中却没有。总之，本研究证明了慢性渗透刺激导致 NG2 和 PDGFRB 的周细胞表达以及感觉 CVO 中血管通透性的变化，表明血管系统积极参与渗透稳态。版权所有 © 2013 约翰·威利父子有限公司
The blood–brain barrier (BBB) is a barrier that prevents free access of blood‐derived substances to the brain through the tight junctions and maintains a specialized brain environment. Circumventricular organs (CVOs) lack the typical BBB. The fenestrated vasculature of the sensory CVOs, including the organum vasculosum of the lamina terminalis (OVLT), subfornical organ (SFO) and area postrema (AP), allows parenchyma cells to sense a variety of blood‐derived information, including osmotic ones. In the present study, we utilized immunohistochemistry to examine changes in the expression of NG2 and platelet‐derived growth factor receptor beta (PDGFRB) in the OVLT, SFO and AP of adult mice during chronic osmotic stimulation. The expression of NG2 and PDGFRB was remarkably prominent in pericytes, although these angiogenesis‐associated proteins are highly expressed at pericytes of developing immature vasculature. The chronic salt loading prominently increased the expression of NG2 in the OVLT and SFO and that of PDGFRB in the OVLT, SFO and AP. The vascular permeability of low‐molecular‐mass tracer fluorescein isothiocyanate was increased significantly by chronic salt loading in the OVLT and SFO but not AP. In conclusion, the present study demonstrates changes in pericyte expression of NG2 and PDGFRB and vascular permeability in the sensory CVOs by chronic osmotic stimulation, indicating active participation of the vascular system in osmotic homeostasis. Copyright © 2013 John Wiley & Sons, Ltd.